Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3159481..3160168 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag HKBT2_RS15070 Protein ID WP_003414624.1
Coordinates 3159725..3160168 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag HKBT2_RS15065 Protein ID WP_003414620.1
Coordinates 3159481..3159738 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS15045 3154801..3155655 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
HKBT2_RS15050 3155711..3156874 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
HKBT2_RS15055 3156891..3158105 - 1215 WP_003414610.1 zinc metalloprotease Rip -
HKBT2_RS15060 3158113..3159354 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
HKBT2_RS15065 3159481..3159738 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
HKBT2_RS15070 3159725..3160168 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBT2_RS15075 3160248..3160910 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
HKBT2_RS15080 3161006..3161197 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HKBT2_RS15085 3161529..3163277 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
HKBT2_RS15090 3163373..3163954 + 582 WP_003414644.1 fasciclin domain-containing protein -
HKBT2_RS15095 3164054..3164320 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T44709 WP_003414624.1 NZ_CP002882:3159725-3160168 [Mycobacterium tuberculosis BT2]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T44709 NZ_CP002882:3159725-3160168 [Mycobacterium tuberculosis BT2]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT44709 WP_003414620.1 NZ_CP002882:3159481-3159738 [Mycobacterium tuberculosis BT2]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT44709 NZ_CP002882:3159481-3159738 [Mycobacterium tuberculosis BT2]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References