44705

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3091461..3092031 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag HKBT2_RS14700 Protein ID WP_003414166.1
Coordinates 3091461..3091817 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag HKBT2_RS14705 Protein ID WP_003901465.1
Coordinates 3091801..3092031 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS14680 3086916..3088604 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
HKBT2_RS14685 3088608..3088934 - 327 WP_003414157.1 hypothetical protein -
HKBT2_RS14690 3089065..3089691 + 627 WP_025329640.1 DUF3558 family protein -
HKBT2_RS14695 3089710..3091359 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
HKBT2_RS14700 3091461..3091817 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
HKBT2_RS14705 3091801..3092031 - 231 WP_003901465.1 antitoxin MazE Antitoxin
HKBT2_RS14710 3092074..3093117 - 1044 WP_025329641.1 DUF2293 domain-containing protein -
HKBT2_RS22260 3093308..3093583 + 276 WP_003911993.1 DUF1778 domain-containing protein -
HKBT2_RS22645 3093759..3094013 - 255 WP_003917684.1 hypothetical protein -
HKBT2_RS14725 3094161..3094565 + 405 WP_003414181.1 hypothetical protein -
HKBT2_RS14730 3094562..3094753 + 192 WP_003414184.1 hypothetical protein -
HKBT2_RS14735 3094817..3096106 + 1290 Protein_2889 transposase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3091461..3104277 12816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T44705 WP_003414166.1 NZ_CP002882:c3091817-3091461 [Mycobacterium tuberculosis BT2]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T44705 NZ_CP002882:c3091817-3091461 [Mycobacterium tuberculosis BT2]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT44705 WP_003901465.1 NZ_CP002882:c3092031-3091801 [Mycobacterium tuberculosis BT2]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT44705 NZ_CP002882:c3092031-3091801 [Mycobacterium tuberculosis BT2]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References