Detailed information of TA system TA044703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3051467..3052146
• Replicon: chromosome
• Accession: NZ_CP002882
• Organism: Mycobacterium tuberculosis BT2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: HKBT2_RS14480
• Protein ID: WP_003414059.1
• Coordinates: 3051467..3051883 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: HKBT2_RS14485
• Protein ID: WP_003414061.1
• Coordinates: 3051880..3052146 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT2_RS14460	3047519..3048421	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HKBT2_RS14465	3048490..3049242	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
HKBT2_RS14470	3049486..3049761	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
HKBT2_RS14475	3049758..3051380	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
HKBT2_RS14480	3051467..3051883	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
HKBT2_RS14485	3051880..3052146	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBT2_RS14490	3052172..3052567	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
HKBT2_RS14495	3052564..3052833	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
HKBT2_RS14500	3052843..3053937	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
HKBT2_RS14505	3053934..3054353	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
HKBT2_RS14510	3054427..3054906	-	480	WP_003414073.1	dihydrofolate reductase	-
HKBT2_RS14515	3054977..3055777	-	801	WP_003911953.1	thymidylate synthase	-
HKBT2_RS14520	3055933..3056670	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T44703 WP_003414059.1 NZ_CP002882:c3051883-3051467 [Mycobacterium tuberculosis BT2]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T44703 NZ_CP002882:c3051883-3051467 [Mycobacterium tuberculosis BT2]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT44703 WP_003414061.1 NZ_CP002882:c3052146-3051880 [Mycobacterium tuberculosis BT2]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT44703 NZ_CP002882:c3052146-3051880 [Mycobacterium tuberculosis BT2]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64