Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2851925..2852562 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag HKBT2_RS13420 Protein ID WP_003413180.1
Coordinates 2851925..2852320 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag HKBT2_RS13425 Protein ID WP_003413183.1
Coordinates 2852317..2852562 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS13385 2847329..2848540 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
HKBT2_RS13390 2848667..2849326 + 660 WP_003902296.1 hypothetical protein -
HKBT2_RS13395 2849323..2849985 + 663 WP_003905878.1 hypothetical protein -
HKBT2_RS22245 2849982..2850259 + 278 Protein_2619 type II toxin-antitoxin system VapB family antitoxin -
HKBT2_RS13400 2850352..2850765 + 414 WP_003413164.1 PIN domain nuclease -
HKBT2_RS13405 2850804..2851061 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
HKBT2_RS13410 2851058..2851435 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
HKBT2_RS13415 2851451..2851825 - 375 WP_003413177.1 hypothetical protein -
HKBT2_RS13420 2851925..2852320 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
HKBT2_RS13425 2852317..2852562 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
HKBT2_RS13430 2852973..2853392 - 420 WP_003413190.1 A24 family peptidase -
HKBT2_RS13435 2853404..2854213 - 810 WP_003413193.1 shikimate dehydrogenase -
HKBT2_RS13440 2854210..2855463 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
HKBT2_RS13445 2855456..2855968 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T44700 WP_003413180.1 NZ_CP002882:c2852320-2851925 [Mycobacterium tuberculosis BT2]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T44700 NZ_CP002882:c2852320-2851925 [Mycobacterium tuberculosis BT2]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT44700 WP_003413183.1 NZ_CP002882:c2852562-2852317 [Mycobacterium tuberculosis BT2]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT44700 NZ_CP002882:c2852562-2852317 [Mycobacterium tuberculosis BT2]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References