Detailed information of TA system TA044699

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2850804..2851435
• Replicon: chromosome
• Accession: NZ_CP002882
• Organism: Mycobacterium tuberculosis BT2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: HKBT2_RS13410
• Protein ID: WP_003413174.1
• Coordinates: 2851058..2851435 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: HKBT2_RS13405
• Protein ID: WP_003413167.1
• Coordinates: 2850804..2851061 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT2_RS13380	2846719..2847033	+	315	WP_009937839.1	hypothetical protein	-
HKBT2_RS13385	2847329..2848540	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
HKBT2_RS13390	2848667..2849326	+	660	WP_003902296.1	hypothetical protein	-
HKBT2_RS13395	2849323..2849985	+	663	WP_003905878.1	hypothetical protein	-
HKBT2_RS22245	2849982..2850259	+	278	Protein_2619	type II toxin-antitoxin system VapB family antitoxin	-
HKBT2_RS13400	2850352..2850765	+	414	WP_003413164.1	PIN domain nuclease	-
HKBT2_RS13405	2850804..2851061	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
HKBT2_RS13410	2851058..2851435	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT2_RS13415	2851451..2851825	-	375	WP_003413177.1	hypothetical protein	-
HKBT2_RS13420	2851925..2852320	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
HKBT2_RS13425	2852317..2852562	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
HKBT2_RS13430	2852973..2853392	-	420	WP_003413190.1	A24 family peptidase	-
HKBT2_RS13435	2853404..2854213	-	810	WP_003413193.1	shikimate dehydrogenase	-
HKBT2_RS13440	2854210..2855463	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
HKBT2_RS13445	2855456..2855968	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T44699 WP_003413174.1 NZ_CP002882:2851058-2851435 [Mycobacterium tuberculosis BT2]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T44699 NZ_CP002882:2851058-2851435 [Mycobacterium tuberculosis BT2]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT44699 WP_003413167.1 NZ_CP002882:2850804-2851061 [Mycobacterium tuberculosis BT2]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT44699 NZ_CP002882:2850804-2851061 [Mycobacterium tuberculosis BT2]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5