Detailed information of TA system TA044697

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2836474..2837114
• Replicon: chromosome
• Accession: NZ_CP002882
• Organism: Mycobacterium tuberculosis BT2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: HKBT2_RS13320
• Protein ID: WP_003412970.1
• Coordinates: 2836474..2836893 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: HKBT2_RS13325
• Protein ID: WP_003412975.1
• Coordinates: 2836890..2837114 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT2_RS13295	2832064..2832786	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
HKBT2_RS13300	2833303..2833525	+	223	Protein_2598	type II toxin-antitoxin system VapB family antitoxin	-
HKBT2_RS13305	2833522..2833923	+	402	WP_003412963.1	PIN domain-containing protein	-
HKBT2_RS13310	2833958..2834878	-	921	WP_003412965.1	restriction endonuclease	-
HKBT2_RS22615	2835219..2835464	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HKBT2_RS13315	2835523..2836473	+	951	WP_003911916.1	Lsr2 family protein	-
HKBT2_RS13320	2836474..2836893	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT2_RS13325	2836890..2837114	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
HKBT2_RS13330	2837145..2839980	-	2836	Protein_2605	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
HKBT2_RS13335	2840052..2840453	-	402	WP_003412981.1	hypothetical protein	-
HKBT2_RS13340	2840453..2840923	-	471	WP_003899364.1	transcription antitermination factor NusB	-
HKBT2_RS13345	2840926..2841489	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T44697 WP_003412970.1 NZ_CP002882:c2836893-2836474 [Mycobacterium tuberculosis BT2]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T44697 NZ_CP002882:c2836893-2836474 [Mycobacterium tuberculosis BT2]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT44697 WP_003412975.1 NZ_CP002882:c2837114-2836890 [Mycobacterium tuberculosis BT2]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT44697 NZ_CP002882:c2837114-2836890 [Mycobacterium tuberculosis BT2]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09