Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2217890..2218476 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag HKBT2_RS10405 Protein ID WP_003410010.1
Coordinates 2217890..2218234 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag HKBT2_RS10410 Protein ID WP_003410014.1
Coordinates 2218228..2218476 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS10370 2213596..2214195 + 600 WP_003910883.1 L-lysine exporter -
HKBT2_RS10375 2214611..2215039 + 429 WP_003409992.1 cellulose-binding protein -
HKBT2_RS10380 2215265..2215804 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
HKBT2_RS10385 2216324..2216884 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
HKBT2_RS10390 2216881..2217222 - 342 WP_003410003.1 DUF2384 domain-containing protein -
HKBT2_RS10395 2217308..2217565 + 258 WP_003410006.1 hypothetical protein -
HKBT2_RS10400 2217466..2217801 - 336 WP_003410009.1 dehydrogenase -
HKBT2_RS10405 2217890..2218234 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
HKBT2_RS10410 2218228..2218476 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
HKBT2_RS10415 2218576..2220891 - 2316 WP_003899120.1 cation transporter ATPase CptG -
HKBT2_RS10420 2220888..2221160 - 273 WP_003410017.1 DUF1490 family protein -
HKBT2_RS10425 2221213..2221569 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
HKBT2_RS22195 2221726..2221830 + 105 Protein_2046 hemerythrin domain-containing protein -
HKBT2_RS10430 2221830..2222642 + 813 Protein_2047 HAD-IC family P-type ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T44690 WP_003410010.1 NZ_CP002882:c2218234-2217890 [Mycobacterium tuberculosis BT2]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T44690 NZ_CP002882:c2218234-2217890 [Mycobacterium tuberculosis BT2]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT44690 WP_003410014.1 NZ_CP002882:c2218476-2218228 [Mycobacterium tuberculosis BT2]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT44690 NZ_CP002882:c2218476-2218228 [Mycobacterium tuberculosis BT2]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References