Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2208998..2209686 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag HKBT2_RS10340 Protein ID WP_003409958.1
Coordinates 2208998..2209417 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag HKBT2_RS10345 Protein ID WP_003409968.1
Coordinates 2209426..2209686 (-) Length 87 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS10320 2204493..2205341 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
HKBT2_RS10325 2205304..2206749 - 1446 WP_003899115.1 APC family permease -
HKBT2_RS10330 2206928..2207614 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
HKBT2_RS10335 2207805..2208773 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
HKBT2_RS10340 2208998..2209417 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBT2_RS10345 2209426..2209686 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HKBT2_RS10350 2209829..2211505 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
HKBT2_RS10355 2211493..2212146 - 654 WP_003409976.1 cutinase Cfp21 -
HKBT2_RS10360 2212261..2212491 + 231 WP_003409980.1 DUF167 domain-containing protein -
HKBT2_RS10365 2212576..2213487 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
HKBT2_RS10370 2213596..2214195 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-125)

Antitoxin

(2-81)


Sequences


Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T44688 WP_003409958.1 NZ_CP002882:c2209417-2208998 [Mycobacterium tuberculosis BT2]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T44688 NZ_CP002882:c2209417-2208998 [Mycobacterium tuberculosis BT2]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT44688 WP_003409968.1 NZ_CP002882:c2209686-2209426 [Mycobacterium tuberculosis BT2]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT44688 NZ_CP002882:c2209686-2209426 [Mycobacterium tuberculosis BT2]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829C4H9


Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References