Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1944019..1944632 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag HKBT2_RS09040 Protein ID WP_003408465.1
Coordinates 1944019..1944408 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag HKBT2_RS09045 Protein ID WP_003408469.1
Coordinates 1944405..1944632 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS09005 1939648..1940562 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
HKBT2_RS09010 1940565..1941395 + 831 WP_003408448.1 cyclase family protein -
HKBT2_RS09015 1941395..1941745 + 351 WP_003898986.1 cupin domain-containing protein -
HKBT2_RS09020 1941798..1942616 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
HKBT2_RS09025 1942630..1943409 + 780 WP_003408460.1 IclR family transcriptional regulator -
HKBT2_RS09040 1944019..1944408 - 390 WP_003408465.1 PIN domain-containing protein Toxin
HKBT2_RS09045 1944405..1944632 - 228 WP_003408469.1 antitoxin Antitoxin
HKBT2_RS09050 1944850..1946334 + 1485 WP_003408473.1 carboxylase -
HKBT2_RS09055 1946331..1947578 + 1248 WP_003408476.1 serine hydrolase -
HKBT2_RS09060 1947621..1948040 - 420 WP_003408483.1 hypothetical protein -
HKBT2_RS09065 1948030..1948740 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T44683 WP_003408465.1 NZ_CP002882:c1944408-1944019 [Mycobacterium tuberculosis BT2]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T44683 NZ_CP002882:c1944408-1944019 [Mycobacterium tuberculosis BT2]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT44683 WP_003408469.1 NZ_CP002882:c1944632-1944405 [Mycobacterium tuberculosis BT2]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT44683 NZ_CP002882:c1944632-1944405 [Mycobacterium tuberculosis BT2]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References