Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1692477..1693093 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag HKBT2_RS07960 Protein ID WP_003407593.1
Coordinates 1692776..1693093 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag HKBT2_RS07955 Protein ID WP_003900349.1
Coordinates 1692477..1692779 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS07945 1688363..1690210 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
HKBT2_RS07950 1690211..1692463 + 2253 WP_025329619.1 methylmalonyl-CoA mutase -
HKBT2_RS07955 1692477..1692779 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
HKBT2_RS07960 1692776..1693093 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
HKBT2_RS07965 1693090..1694094 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
HKBT2_RS07970 1694147..1695436 + 1290 WP_025329620.1 serine hydrolase -
HKBT2_RS07975 1695509..1696282 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
HKBT2_RS07980 1696340..1696552 - 213 WP_003898900.1 dodecin family protein -
HKBT2_RS22530 1696562..1696786 - 225 WP_160522526.1 NUDIX domain-containing protein -
HKBT2_RS07990 1697056..1698084 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T44681 WP_003407593.1 NZ_CP002882:1692776-1693093 [Mycobacterium tuberculosis BT2]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T44681 NZ_CP002882:1692776-1693093 [Mycobacterium tuberculosis BT2]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT44681 WP_003900349.1 NZ_CP002882:1692477-1692779 [Mycobacterium tuberculosis BT2]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT44681 NZ_CP002882:1692477-1692779 [Mycobacterium tuberculosis BT2]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References