Detailed information of TA system TA044676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239654..1240222
• Replicon: chromosome
• Accession: NZ_CP002882
• Organism: Mycobacterium tuberculosis BT2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: HKBT2_RS05915
• Protein ID: WP_003405865.1
• Coordinates: 1239848..1240222 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HKBT2_RS05910
• Protein ID: WP_003405863.1
• Coordinates: 1239654..1239851 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT2_RS05890	1235695..1236333	-	639	WP_003898730.1	lipid droplet-associated protein	-
HKBT2_RS05895	1236423..1237430	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HKBT2_RS05900	1237447..1238430	-	984	WP_003898731.1	hypothetical protein	-
HKBT2_RS05905	1238493..1239566	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HKBT2_RS05910	1239654..1239851	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBT2_RS05915	1239848..1240222	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT2_RS05920	1240425..1241123	+	699	WP_003898733.1	hypothetical protein	-
HKBT2_RS05925	1241241..1241426	+	186	WP_003901093.1	hypothetical protein	-
HKBT2_RS05930	1241353..1241628	-	276	WP_003405867.1	hypothetical protein	-
HKBT2_RS05935	1241871..1242194	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
HKBT2_RS05940	1242209..1242919	-	711	WP_003911399.1	hypothetical protein	-
HKBT2_RS22505	1242941..1243150	+	210	WP_003911400.1	hypothetical protein	-
HKBT2_RS05950	1243102..1243809	-	708	Protein_1163	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T44676 WP_003405865.1 NZ_CP002882:1239848-1240222 [Mycobacterium tuberculosis BT2]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T44676 NZ_CP002882:1239848-1240222 [Mycobacterium tuberculosis BT2]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT44676 WP_003405863.1 NZ_CP002882:1239654-1239851 [Mycobacterium tuberculosis BT2]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT44676 NZ_CP002882:1239654-1239851 [Mycobacterium tuberculosis BT2]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07