Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230898..1231529 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag HKBT2_RS05855 Protein ID WP_003405820.1
Coordinates 1230898..1231209 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag HKBT2_RS05860 Protein ID WP_003405836.1
Coordinates 1231209..1231529 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS05835 1226379..1227803 - 1425 WP_003405805.1 class II fumarate hydratase -
HKBT2_RS05840 1227834..1228922 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
HKBT2_RS05845 1228978..1229622 + 645 WP_003915494.1 DUF4245 domain-containing protein -
HKBT2_RS05850 1229629..1230786 - 1158 WP_003898727.1 AI-2E family transporter -
HKBT2_RS05855 1230898..1231209 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
HKBT2_RS05860 1231209..1231529 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
HKBT2_RS21035 1231539..1233064 + 1526 Protein_1147 carboxylesterase/lipase family protein -
HKBT2_RS05875 1233082..1234194 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
HKBT2_RS05880 1234204..1234461 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
HKBT2_RS05885 1234451..1235698 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
HKBT2_RS05890 1235695..1236333 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T44675 WP_003405820.1 NZ_CP002882:c1231209-1230898 [Mycobacterium tuberculosis BT2]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T44675 NZ_CP002882:c1231209-1230898 [Mycobacterium tuberculosis BT2]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT44675 WP_003405836.1 NZ_CP002882:c1231529-1231209 [Mycobacterium tuberculosis BT2]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT44675 NZ_CP002882:c1231529-1231209 [Mycobacterium tuberculosis BT2]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References