Detailed information of TA system TA044670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-mazE/PIN(toxin)
• Location: 753987..754779
• Replicon: chromosome
• Accession: NZ_CP002882
• Organism: Mycobacterium tuberculosis BT2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WFB2
• Locus tag: HKBT2_RS03460
• Protein ID: WP_003403386.1
• Coordinates: 754342..754779 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TQE0
• Locus tag: HKBT2_RS03455
• Protein ID: WP_003403381.1
• Coordinates: 753987..754232 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBT2_RS03425	749007..750512	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
HKBT2_RS03430	750593..751603	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
HKBT2_RS03435	751991..752374	-	384	WP_003403365.1	ribonuclease VapC6	-
HKBT2_RS03440	752469..752624	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	-
HKBT2_RS03445	752700..753416	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
HKBT2_RS03450	753692..754000	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
HKBT2_RS03455	753987..754232	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	Antitoxin
HKBT2_RS03460	754342..754779	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBT2_RS03465	754776..755030	-	255	WP_003911263.1	antitoxin VapB7	-
HKBT2_RS03470	755144..757507	+	2364	WP_003901895.1	arylsulfatase AtsD	-
HKBT2_RS03475	757570..757896	+	327	WP_003403401.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HKBT2_RS03480	757808..758146	+	339	WP_003403405.1	PIN domain-containing protein	-
HKBT2_RS03485	758143..758316	+	174	WP_003898549.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-126)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15245.39 Da
• Isoelectric Point: 5.1865

>T44670 WP_003403386.1 NZ_CP002882:c754779-754342 [Mycobacterium tuberculosis BT2]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Nucleotide

• Length: 438 bp

>T44670 NZ_CP002882:c754779-754342 [Mycobacterium tuberculosis BT2]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9169.01 Da
• Isoelectric Point: 4.2070

>AT44670 WP_003403381.1 NZ_CP002882:c754232-753987 [Mycobacterium tuberculosis BT2]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Nucleotide

• Length: 246 bp

>AT44670 NZ_CP002882:c754232-753987 [Mycobacterium tuberculosis BT2]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BSW5

Antitoxin

• AlphaFold DB: G0TQE0