Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/AbrB(antitoxin)
Location 695608..696254 Replicon chromosome
Accession NZ_CP002882
Organism Mycobacterium tuberculosis BT2

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ88
Locus tag HKBT2_RS03105 Protein ID WP_003403137.1
Coordinates 695608..696021 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ89
Locus tag HKBT2_RS03110 Protein ID WP_003403139.1
Coordinates 696018..696254 (-) Length 79 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBT2_RS03085 691691..693241 + 1551 WP_003905349.1 MCE family protein -
HKBT2_RS03090 693293..693685 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 -
HKBT2_RS03095 693682..693939 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 -
HKBT2_RS03100 694122..695357 - 1236 WP_003403128.1 ATP-binding protein -
HKBT2_RS03105 695608..696021 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBT2_RS03110 696018..696254 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HKBT2_RS03115 696358..696864 - 507 WP_003900973.1 two component system sensor kinase HK1 -
HKBT2_RS03120 696978..697508 - 531 WP_003917320.1 two component system sensor kinase HK2 -
HKBT2_RS03125 697492..698253 - 762 WP_003403148.1 two-component system response regulator TcrA -
HKBT2_RS03130 698310..698621 + 312 WP_003403164.1 hypothetical protein -
HKBT2_RS03135 698693..699643 + 951 WP_003900184.1 DUF1259 domain-containing protein -
HKBT2_RS03140 699884..700468 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
HKBT2_RS03145 700470..701180 + 711 Protein_624 transposase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(7-126)

Antitoxin

(3-43)


Sequences


Toxin        


Download         Length: 138 a.a.        Molecular weight: 14786.14 Da        Isoelectric Point: 8.1405

>T44663 WP_003403137.1 NZ_CP002882:c696021-695608 [Mycobacterium tuberculosis BT2]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Download         Length: 414 bp

>T44663 NZ_CP002882:c696021-695608 [Mycobacterium tuberculosis BT2]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8212.41 Da        Isoelectric Point: 5.0351

>AT44663 WP_003403139.1 NZ_CP002882:c696254-696018 [Mycobacterium tuberculosis BT2]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Download         Length: 237 bp

>AT44663 NZ_CP002882:c696254-696018 [Mycobacterium tuberculosis BT2]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G0TQ88


Antitoxin

Source ID Structure
AlphaFold DB G0TQ89

References