Detailed information of TA system TA044651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3695996..3696670
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: HKBS1_RS17500
• Protein ID: WP_003417282.1
• Coordinates: 3695996..3696424 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: HKBS1_RS17505
• Protein ID: WP_003417286.1
• Coordinates: 3696428..3696670 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS17475	3691818..3692219	-	402	WP_003417264.1	cytidine deaminase	-
HKBS1_RS17480	3692360..3692794	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
HKBS1_RS17485	3692791..3693225	+	435	WP_003902441.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
HKBS1_RS17490	3693354..3695126	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
HKBS1_RS17495	3695126..3695917	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
HKBS1_RS17500	3695996..3696424	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBS1_RS17505	3696428..3696670	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
HKBS1_RS17510	3696792..3697406	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
HKBS1_RS17515	3697403..3698068	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
HKBS1_RS17520	3698069..3698602	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
HKBS1_RS17525	3698599..3698973	-	375	WP_003417295.1	4a-hydroxytetrahydrobiopterin dehydratase	-
HKBS1_RS17530	3699070..3700134	-	1065	WP_003913037.1	GTP 3',8-cyclase MoaA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T44651 WP_003417282.1 NZ_CP002871:c3696424-3695996 [Mycobacterium tuberculosis HKBS1]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T44651 NZ_CP002871:c3696424-3695996 [Mycobacterium tuberculosis HKBS1]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT44651 WP_003417286.1 NZ_CP002871:c3696670-3696428 [Mycobacterium tuberculosis HKBS1]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT44651 NZ_CP002871:c3696670-3696428 [Mycobacterium tuberculosis HKBS1]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59