Detailed information of TA system TA044647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3121224..3121828
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: HKBS1_RS14860
• Protein ID: WP_003414492.1
• Coordinates: 3121224..3121616 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: HKBS1_RS14865
• Protein ID: WP_003414495.1
• Coordinates: 3121613..3121828 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS14830	3116374..3117162	-	789	WP_003917092.1	CRISPR-associated endoribonuclease Cas6	-
HKBS1_RS14835	3117496..3118041	-	546	WP_014584866.1	DUF1802 family protein	-
HKBS1_RS14840	3118313..3119197	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HKBS1_RS14845	3119200..3120087	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
HKBS1_RS14850	3120392..3120937	-	546	WP_003910939.1	DUF1802 family protein	-
HKBS1_RS14855	3120934..3121203	-	270	WP_003414489.1	DUF2277 family protein	-
HKBS1_RS14860	3121224..3121616	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBS1_RS14865	3121613..3121828	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HKBS1_RS14870	3121875..3122624	+	750	WP_003902358.1	enoyl-CoA hydratase	-
HKBS1_RS14875	3122703..3123785	-	1083	WP_003414499.1	ABC transporter ATP-binding protein	-
HKBS1_RS14880	3123778..3125088	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
HKBS1_RS14885	3125091..3125918	-	828	WP_003414504.1	carbohydrate ABC transporter permease	-
HKBS1_RS14890	3125915..3126826	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T44647 WP_003414492.1 NZ_CP002871:c3121616-3121224 [Mycobacterium tuberculosis HKBS1]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T44647 NZ_CP002871:c3121616-3121224 [Mycobacterium tuberculosis HKBS1]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT44647 WP_003414495.1 NZ_CP002871:c3121828-3121613 [Mycobacterium tuberculosis HKBS1]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT44647 NZ_CP002871:c3121828-3121613 [Mycobacterium tuberculosis HKBS1]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8