Detailed information of TA system TA044643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3061029..3061708
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: HKBS1_RS14510
• Protein ID: WP_003414059.1
• Coordinates: 3061029..3061445 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HKBS1_RS14515
• Protein ID: WP_025234880.1
• Coordinates: 3061442..3061708 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS14490	3057081..3057983	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HKBS1_RS14495	3058052..3058804	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
HKBS1_RS14500	3059048..3059323	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
HKBS1_RS14505	3059320..3060942	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
HKBS1_RS14510	3061029..3061445	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
HKBS1_RS14515	3061442..3061708	-	267	WP_025234880.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBS1_RS14520	3061734..3062129	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
HKBS1_RS14525	3062126..3062395	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
HKBS1_RS14530	3062405..3063499	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
HKBS1_RS14535	3063496..3063915	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
HKBS1_RS14540	3063989..3064468	-	480	WP_003414073.1	dihydrofolate reductase	-
HKBS1_RS14545	3064539..3065339	-	801	WP_003911953.1	thymidylate synthase	-
HKBS1_RS14550	3065495..3066232	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T44643 WP_003414059.1 NZ_CP002871:c3061445-3061029 [Mycobacterium tuberculosis HKBS1]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T44643 NZ_CP002871:c3061445-3061029 [Mycobacterium tuberculosis HKBS1]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9592.91 Da
• Isoelectric Point: 8.0753

>AT44643 WP_025234880.1 NZ_CP002871:c3061708-3061442 [Mycobacterium tuberculosis HKBS1]
MHRRYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT44643 NZ_CP002871:c3061708-3061442 [Mycobacterium tuberculosis HKBS1]
ATGCATCGCAGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2