Detailed information of TA system TA044637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2846236..2846876
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: HKBS1_RS13350
• Protein ID: WP_003412970.1
• Coordinates: 2846236..2846655 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: HKBS1_RS13355
• Protein ID: WP_003412975.1
• Coordinates: 2846652..2846876 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS13325	2841826..2842548	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
HKBS1_RS13330	2843065..2843287	+	223	Protein_2605	type II toxin-antitoxin system VapB family antitoxin	-
HKBS1_RS13335	2843284..2843685	+	402	WP_003412963.1	PIN domain-containing protein	-
HKBS1_RS13340	2843720..2844640	-	921	WP_003412965.1	restriction endonuclease	-
HKBS1_RS22600	2844981..2845226	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HKBS1_RS13345	2845285..2846235	+	951	WP_003911916.1	Lsr2 family protein	-
HKBS1_RS13350	2846236..2846655	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBS1_RS13355	2846652..2846876	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
HKBS1_RS13360	2846907..2849750	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
HKBS1_RS13365	2849822..2850223	-	402	WP_003412981.1	hypothetical protein	-
HKBS1_RS13370	2850223..2850693	-	471	WP_003899364.1	transcription antitermination factor NusB	-
HKBS1_RS13375	2850696..2851259	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T44637 WP_003412970.1 NZ_CP002871:c2846655-2846236 [Mycobacterium tuberculosis HKBS1]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T44637 NZ_CP002871:c2846655-2846236 [Mycobacterium tuberculosis HKBS1]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT44637 WP_003412975.1 NZ_CP002871:c2846876-2846652 [Mycobacterium tuberculosis HKBS1]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT44637 NZ_CP002871:c2846876-2846652 [Mycobacterium tuberculosis HKBS1]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09