Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2800057..2800709 Replicon chromosome
Accession NZ_CP002871
Organism Mycobacterium tuberculosis HKBS1

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag HKBS1_RS13150 Protein ID WP_003905869.1
Coordinates 2800284..2800709 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag HKBS1_RS13145 Protein ID WP_003412749.1
Coordinates 2800057..2800278 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBS1_RS13130 2798297..2798503 - 207 WP_003899347.1 hypothetical protein -
HKBS1_RS13135 2798639..2799262 + 624 WP_003900858.1 TIGR00725 family protein -
HKBS1_RS13140 2799252..2800004 + 753 WP_003901416.1 hypothetical protein -
HKBS1_RS13145 2800057..2800278 + 222 WP_003412749.1 antitoxin Antitoxin
HKBS1_RS13150 2800284..2800709 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBS1_RS13155 2800732..2801913 - 1182 WP_003905870.1 2-oxo acid dehydrogenase subunit E2 -
HKBS1_RS13160 2801910..2802956 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
HKBS1_RS13165 2802967..2804070 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
HKBS1_RS13170 2804329..2805150 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
HKBS1_RS13175 2805147..2805704 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T44636 WP_003905869.1 NZ_CP002871:2800284-2800709 [Mycobacterium tuberculosis HKBS1]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T44636 NZ_CP002871:2800284-2800709 [Mycobacterium tuberculosis HKBS1]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT44636 WP_003412749.1 NZ_CP002871:2800057-2800278 [Mycobacterium tuberculosis HKBS1]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT44636 NZ_CP002871:2800057-2800278 [Mycobacterium tuberculosis HKBS1]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References