Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2223947..2224533 Replicon chromosome
Accession NZ_CP002871
Organism Mycobacterium tuberculosis HKBS1

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag HKBS1_RS10420 Protein ID WP_003410010.1
Coordinates 2223947..2224291 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag HKBS1_RS10425 Protein ID WP_003410014.1
Coordinates 2224285..2224533 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBS1_RS10385 2219653..2220252 + 600 WP_003910883.1 L-lysine exporter -
HKBS1_RS10390 2220668..2221096 + 429 WP_003409992.1 cellulose-binding protein -
HKBS1_RS10395 2221322..2221861 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
HKBS1_RS10400 2222381..2222941 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
HKBS1_RS10405 2222938..2223279 - 342 WP_003410003.1 DUF2384 domain-containing protein -
HKBS1_RS10410 2223365..2223622 + 258 WP_003410006.1 hypothetical protein -
HKBS1_RS10415 2223523..2223858 - 336 WP_003410009.1 dehydrogenase -
HKBS1_RS10420 2223947..2224291 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
HKBS1_RS10425 2224285..2224533 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
HKBS1_RS10430 2224633..2226948 - 2316 WP_003899120.1 cation transporter ATPase CptG -
HKBS1_RS10435 2226945..2227217 - 273 WP_003410017.1 DUF1490 family protein -
HKBS1_RS10440 2227270..2227626 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
HKBS1_RS10445 2227783..2228550 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T44630 WP_003410010.1 NZ_CP002871:c2224291-2223947 [Mycobacterium tuberculosis HKBS1]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T44630 NZ_CP002871:c2224291-2223947 [Mycobacterium tuberculosis HKBS1]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT44630 WP_003410014.1 NZ_CP002871:c2224533-2224285 [Mycobacterium tuberculosis HKBS1]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT44630 NZ_CP002871:c2224533-2224285 [Mycobacterium tuberculosis HKBS1]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References