Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2215055..2215743 Replicon chromosome
Accession NZ_CP002871
Organism Mycobacterium tuberculosis HKBS1

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag HKBS1_RS10355 Protein ID WP_003409958.1
Coordinates 2215055..2215474 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag HKBS1_RS10360 Protein ID WP_003409968.1
Coordinates 2215483..2215743 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKBS1_RS10335 2210550..2211398 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
HKBS1_RS10340 2211361..2212806 - 1446 WP_003899115.1 APC family permease -
HKBS1_RS10345 2212985..2213671 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
HKBS1_RS10350 2213862..2214830 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
HKBS1_RS10355 2215055..2215474 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
HKBS1_RS10360 2215483..2215743 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HKBS1_RS10365 2215886..2217562 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
HKBS1_RS10370 2217550..2218203 - 654 WP_003409976.1 cutinase Cfp21 -
HKBS1_RS10375 2218318..2218548 + 231 WP_003409980.1 DUF167 domain-containing protein -
HKBS1_RS10380 2218633..2219544 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
HKBS1_RS10385 2219653..2220252 + 600 WP_003910883.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T44628 WP_003409958.1 NZ_CP002871:c2215474-2215055 [Mycobacterium tuberculosis HKBS1]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T44628 NZ_CP002871:c2215474-2215055 [Mycobacterium tuberculosis HKBS1]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT44628 WP_003409968.1 NZ_CP002871:c2215743-2215483 [Mycobacterium tuberculosis HKBS1]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT44628 NZ_CP002871:c2215743-2215483 [Mycobacterium tuberculosis HKBS1]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References