Detailed information of TA system TA044625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2179790..2180493
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: HKBS1_RS10105
• Protein ID: WP_003409778.1
• Coordinates: 2179790..2180119 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: HKBS1_RS21310
• Protein ID: WP_003409780.1
• Coordinates: 2180116..2180493 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS10085	2176173..2177243	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
HKBS1_RS10090	2177240..2177755	+	516	WP_003409718.1	flavin reductase family protein	-
HKBS1_RS10095	2177752..2178813	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
HKBS1_RS10100	2178810..2179580	+	771	WP_003409775.1	SDR family oxidoreductase	-
HKBS1_RS10105	2179790..2180119	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HKBS1_RS21310	2180116..2180493	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
HKBS1_RS10115	2180490..2181080	-	591	WP_003409784.1	SEC-C domain-containing protein	-
HKBS1_RS10120	2181135..2182499	+	1365	WP_003903691.1	HNH endonuclease	-
HKBS1_RS10125	2182654..2183106	-	453	WP_003899095.1	lipoprotein	-
HKBS1_RS10130	2183170..2183571	+	402	WP_025234870.1	hypothetical protein	-
HKBS1_RS10135	2183564..2183746	-	183	WP_003409870.1	hypothetical protein	-
HKBS1_RS10140	2183860..2184210	-	351	WP_003409871.1	hypothetical protein	-
HKBS1_RS10145	2184221..2185123	-	903	WP_003899097.1	hypothetical protein	-
HKBS1_RS10150	2185144..2185335	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T44625 WP_003409778.1 NZ_CP002871:c2180119-2179790 [Mycobacterium tuberculosis HKBS1]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T44625 NZ_CP002871:c2180119-2179790 [Mycobacterium tuberculosis HKBS1]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT44625 WP_003409780.1 NZ_CP002871:c2180493-2180116 [Mycobacterium tuberculosis HKBS1]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT44625 NZ_CP002871:c2180493-2180116 [Mycobacterium tuberculosis HKBS1]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8