Detailed information of TA system TA044622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1766729..1767342
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: HKBS1_RS08285
• Protein ID: WP_003407786.1
• Coordinates: 1766938..1767342 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: HKBS1_RS08280
• Protein ID: WP_009935474.1
• Coordinates: 1766729..1766932 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS08250	1762134..1762514	+	381	WP_003407768.1	fumarate reductase subunit C	-
HKBS1_RS08255	1762511..1762888	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
HKBS1_RS08260	1762956..1763564	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
HKBS1_RS08265	1763748..1764896	+	1149	Protein_1617	MMPL family transporter	-
HKBS1_RS08270	1764906..1765352	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
HKBS1_RS08275	1765387..1766676	+	1290	WP_003407781.1	threonine ammonia-lyase	-
HKBS1_RS08280	1766729..1766932	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBS1_RS08285	1766938..1767342	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
HKBS1_RS08290	1767359..1769101	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
HKBS1_RS08295	1769094..1771391	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T44622 WP_003407786.1 NZ_CP002871:1766938-1767342 [Mycobacterium tuberculosis HKBS1]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T44622 NZ_CP002871:1766938-1767342 [Mycobacterium tuberculosis HKBS1]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT44622 WP_009935474.1 NZ_CP002871:1766729-1766932 [Mycobacterium tuberculosis HKBS1]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT44622 NZ_CP002871:1766729-1766932 [Mycobacterium tuberculosis HKBS1]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46