Detailed information of TA system TA044616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1236794..1237362
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: HKBS1_RS05900
• Protein ID: WP_003405865.1
• Coordinates: 1236988..1237362 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: HKBS1_RS05895
• Protein ID: WP_003405863.1
• Coordinates: 1236794..1236991 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS05875	1232835..1233473	-	639	WP_003898730.1	lipid droplet-associated protein	-
HKBS1_RS05880	1233563..1234570	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
HKBS1_RS05885	1234587..1235570	-	984	WP_003898731.1	hypothetical protein	-
HKBS1_RS05890	1235633..1236706	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
HKBS1_RS05895	1236794..1236991	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HKBS1_RS05900	1236988..1237362	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBS1_RS05905	1237565..1238263	+	699	WP_003898733.1	hypothetical protein	-
HKBS1_RS05910	1238381..1238566	+	186	WP_003901093.1	hypothetical protein	-
HKBS1_RS05915	1238493..1238768	-	276	WP_003405867.1	hypothetical protein	-
HKBS1_RS05920	1239011..1239334	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
HKBS1_RS05925	1239349..1240059	-	711	WP_003911399.1	hypothetical protein	-
HKBS1_RS22490	1240081..1240290	+	210	WP_003911400.1	hypothetical protein	-
HKBS1_RS05935	1240242..1240949	-	708	Protein_1161	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T44616 WP_003405865.1 NZ_CP002871:1236988-1237362 [Mycobacterium tuberculosis HKBS1]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T44616 NZ_CP002871:1236988-1237362 [Mycobacterium tuberculosis HKBS1]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT44616 WP_003405863.1 NZ_CP002871:1236794-1236991 [Mycobacterium tuberculosis HKBS1]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT44616 NZ_CP002871:1236794-1236991 [Mycobacterium tuberculosis HKBS1]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07