Detailed information of TA system TA044615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1228038..1228669
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7U4F9D0
• Locus tag: HKBS1_RS05840
• Protein ID: WP_003405820.1
• Coordinates: 1228038..1228349 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HKBS1_RS05845
• Protein ID: WP_025234854.1
• Coordinates: 1228349..1228669 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS05820	1223519..1224943	-	1425	WP_003405805.1	class II fumarate hydratase	-
HKBS1_RS05825	1224974..1226062	-	1089	WP_003898726.1	class II fructose-bisphosphatase	-
HKBS1_RS05830	1226118..1226762	+	645	WP_003915494.1	DUF4245 domain-containing protein	-
HKBS1_RS05835	1226769..1227926	-	1158	WP_003898727.1	AI-2E family transporter	-
HKBS1_RS05840	1228038..1228349	-	312	WP_003405820.1	type II toxin-antitoxin system toxin endoribonuclease MazF3	Toxin
HKBS1_RS05845	1228349..1228669	-	321	WP_025234854.1	type II toxin-antitoxin system antitoxin MazE3	Antitoxin
HKBS1_RS21040	1228679..1230204	+	1526	Protein_1145	carboxylesterase/lipase family protein	-
HKBS1_RS05860	1230222..1231334	-	1113	WP_003405840.1	3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase	-
HKBS1_RS05865	1231344..1231601	-	258	WP_003405844.1	exodeoxyribonuclease VII small subunit	-
HKBS1_RS05870	1231591..1232838	-	1248	WP_003405846.1	exodeoxyribonuclease VII large subunit	-
HKBS1_RS05875	1232835..1233473	-	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11061.82 Da
• Isoelectric Point: 4.9371

>T44615 WP_003405820.1 NZ_CP002871:c1228349-1228038 [Mycobacterium tuberculosis HKBS1]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Nucleotide

• Length: 312 bp

>T44615 NZ_CP002871:c1228349-1228038 [Mycobacterium tuberculosis HKBS1]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11426.89 Da
• Isoelectric Point: 5.1236

>AT44615 WP_025234854.1 NZ_CP002871:c1228669-1228349 [Mycobacterium tuberculosis HKBS1]
MYLPWGVVLAGGANGFGAGAYQTGTICEMSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Nucleotide

• Length: 321 bp

>AT44615 NZ_CP002871:c1228669-1228349 [Mycobacterium tuberculosis HKBS1]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAATGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Structures

Toxin

• AlphaFold DB: A0A7U4F9D0