Detailed information of TA system TA044604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 695527..696173
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: HKBS1_RS03105
• Protein ID: WP_003403137.1
• Coordinates: 695527..695940 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: HKBS1_RS03110
• Protein ID: WP_003403139.1
• Coordinates: 695937..696173 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS03085	691610..693160	+	1551	WP_003905349.1	MCE family protein	-
HKBS1_RS03090	693212..693604	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
HKBS1_RS03095	693601..693858	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
HKBS1_RS03100	694041..695276	-	1236	WP_003403128.1	ATP-binding protein	-
HKBS1_RS22440	695393..695566	+	174	WP_003403133.1	hypothetical protein	-
HKBS1_RS03105	695527..695940	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKBS1_RS03110	695937..696173	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HKBS1_RS03115	696277..696783	-	507	WP_003900973.1	two component system sensor kinase HK1	-
HKBS1_RS03120	696897..697427	-	531	WP_003917320.1	two component system sensor kinase HK2	-
HKBS1_RS03125	697411..698172	-	762	WP_003403148.1	two-component system response regulator TcrA	-
HKBS1_RS03130	698229..698540	+	312	WP_003403164.1	hypothetical protein	-
HKBS1_RS03135	698612..699562	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
HKBS1_RS03140	699803..700387	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
HKBS1_RS03145	700389..701099	+	711	Protein_625	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T44604 WP_003403137.1 NZ_CP002871:c695940-695527 [Mycobacterium tuberculosis HKBS1]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T44604 NZ_CP002871:c695940-695527 [Mycobacterium tuberculosis HKBS1]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT44604 WP_003403139.1 NZ_CP002871:c696173-695937 [Mycobacterium tuberculosis HKBS1]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT44604 NZ_CP002871:c696173-695937 [Mycobacterium tuberculosis HKBS1]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89