Detailed information of TA system TA044603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 693212..693858
• Replicon: chromosome
• Accession: NZ_CP002871
• Organism: Mycobacterium tuberculosis HKBS1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O07783
• Locus tag: HKBS1_RS03090
• Protein ID: WP_003403122.1
• Coordinates: 693212..693604 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: HKBS1_RS03095
• Protein ID: WP_003403125.1
• Coordinates: 693601..693858 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKBS1_RS03075	688874..690400	+	1527	WP_003403112.1	virulence factor Mce family protein	-
HKBS1_RS03080	690463..691605	+	1143	WP_003911253.1	virulence factor Mce family protein	-
HKBS1_RS03085	691610..693160	+	1551	WP_003905349.1	MCE family protein	-
HKBS1_RS03090	693212..693604	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
HKBS1_RS03095	693601..693858	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
HKBS1_RS03100	694041..695276	-	1236	WP_003403128.1	ATP-binding protein	-
HKBS1_RS22440	695393..695566	+	174	WP_003403133.1	hypothetical protein	-
HKBS1_RS03105	695527..695940	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
HKBS1_RS03110	695937..696173	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HKBS1_RS03115	696277..696783	-	507	WP_003900973.1	two component system sensor kinase HK1	-
HKBS1_RS03120	696897..697427	-	531	WP_003917320.1	two component system sensor kinase HK2	-
HKBS1_RS03125	697411..698172	-	762	WP_003403148.1	two-component system response regulator TcrA	-
HKBS1_RS03130	698229..698540	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14080.08 Da
• Isoelectric Point: 4.6558

>T44603 WP_003403122.1 NZ_CP002871:c693604-693212 [Mycobacterium tuberculosis HKBS1]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Nucleotide

• Length: 393 bp

>T44603 NZ_CP002871:c693604-693212 [Mycobacterium tuberculosis HKBS1]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT44603 WP_003403125.1 NZ_CP002871:c693858-693601 [Mycobacterium tuberculosis HKBS1]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT44603 NZ_CP002871:c693858-693601 [Mycobacterium tuberculosis HKBS1]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4F8V4

Antitoxin

• AlphaFold DB: G0TQ86