Detailed information of TA system TA044578

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1292609..1293141
• Replicon: chromosome
• Accession: NZ_AP028142
• Organism: Polynucleobacter sp. HIN11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QUE60_RS06730
• Protein ID: WP_286226488.1
• Coordinates: 1292609..1292932 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: QUE60_RS06735
• Protein ID: WP_286226489.1
• Coordinates: 1292929..1293141 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE60_RS06725 (PHIN11_12970)	1288794..1292612	+	3819	WP_286226487.1	magnesium chelatase subunit H	-
QUE60_RS06730 (PHIN11_12980)	1292609..1292932	-	324	WP_286226488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUE60_RS06735 (PHIN11_12990)	1292929..1293141	-	213	WP_286226489.1	antitoxin MazE family protein	Antitoxin
QUE60_RS06740 (PHIN11_13000)	1293226..1294065	-	840	WP_286226490.1	aldo/keto reductase	-
QUE60_RS06745 (PHIN11_13010)	1294073..1294882	-	810	WP_286226491.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• Antitoxin MazE-like (8-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11686.56 Da
• Isoelectric Point: 8.6590

>T44578 WP_286226488.1 NZ_AP028142:c1292932-1292609 [Polynucleobacter sp. HIN11]
VRRGDFVKVVTPGSYGKPRPALIVQSDLFNQHPSVSVLPLSSELRNAPLFRVPIEPSSMNGLEKRSEVMVDKIQTIPVDK
IGGEIGRASDEEMLAINRALAIFLGFA

Nucleotide

• Length: 324 bp

>T44578 NZ_AP028142:c1292932-1292609 [Polynucleobacter sp. HIN11]
GTGAGGCGAGGTGATTTTGTAAAGGTTGTCACACCGGGCTCCTATGGCAAACCAAGACCCGCATTAATCGTTCAATCGGA
TTTATTTAATCAGCATCCATCGGTATCTGTGCTGCCTTTATCAAGTGAACTACGAAACGCTCCTTTGTTTAGGGTACCAA
TTGAACCCTCCTCAATGAATGGTTTAGAGAAACGTTCAGAGGTGATGGTGGACAAAATTCAGACCATACCAGTAGACAAG
ATTGGCGGAGAAATTGGGCGTGCTTCGGATGAAGAAATGCTAGCGATTAATCGTGCGCTGGCAATATTTTTAGGGTTTGC
TTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8317.43 Da
• Isoelectric Point: 8.0273

>AT44578 WP_286226489.1 NZ_AP028142:c1293141-1292929 [Polynucleobacter sp. HIN11]
MPVATNLRVQKHRAQLRAAGLRPIQIWVPDTRQSSFAEECRRQSQMLKNDPLESEINEWLDEVRDTKDWK

Nucleotide

• Length: 213 bp

>AT44578 NZ_AP028142:c1293141-1292929 [Polynucleobacter sp. HIN11]
ATGCCAGTTGCCACTAACTTAAGAGTCCAAAAACACCGGGCGCAATTACGTGCGGCAGGGCTTCGTCCTATCCAAATCTG
GGTTCCAGATACACGCCAATCCTCTTTTGCAGAGGAGTGTCGCCGTCAGTCGCAAATGTTAAAAAATGATCCACTCGAAT
CTGAAATCAATGAGTGGCTTGATGAGGTTCGTGATACCAAGGATTGGAAGTGA