Detailed information of TA system TA044575

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1293120..1293652
• Replicon: chromosome
• Accession: NZ_AP028141
• Organism: Polynucleobacter sp. HIN10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QUE67_RS06745
• Protein ID: WP_286226488.1
• Coordinates: 1293120..1293443 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: QUE67_RS06750
• Protein ID: WP_286226489.1
• Coordinates: 1293440..1293652 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE67_RS06740 (PHIN10_12970)	1289305..1293123	+	3819	WP_286226487.1	magnesium chelatase subunit H	-
QUE67_RS06745 (PHIN10_12980)	1293120..1293443	-	324	WP_286226488.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUE67_RS06750 (PHIN10_12990)	1293440..1293652	-	213	WP_286226489.1	antitoxin MazE family protein	Antitoxin
QUE67_RS06755 (PHIN10_13000)	1293737..1294576	-	840	WP_286226490.1	aldo/keto reductase	-
QUE67_RS06760 (PHIN10_13010)	1294584..1295393	-	810	WP_286226491.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-104)

Antitoxin

• Antitoxin MazE-like (8-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11686.56 Da
• Isoelectric Point: 8.6590

>T44575 WP_286226488.1 NZ_AP028141:c1293443-1293120 [Polynucleobacter sp. HIN10]
VRRGDFVKVVTPGSYGKPRPALIVQSDLFNQHPSVSVLPLSSELRNAPLFRVPIEPSSMNGLEKRSEVMVDKIQTIPVDK
IGGEIGRASDEEMLAINRALAIFLGFA

Nucleotide

• Length: 324 bp

>T44575 NZ_AP028141:c1293443-1293120 [Polynucleobacter sp. HIN10]
GTGAGGCGAGGTGATTTTGTAAAGGTTGTCACACCGGGCTCCTATGGCAAACCAAGACCCGCATTAATCGTTCAATCGGA
TTTATTTAATCAGCATCCATCGGTATCTGTGCTGCCTTTATCAAGTGAACTACGAAACGCTCCTTTGTTTAGGGTACCAA
TTGAACCCTCCTCAATGAATGGTTTAGAGAAACGTTCAGAGGTGATGGTGGACAAAATTCAGACCATACCAGTAGACAAG
ATTGGCGGAGAAATTGGGCGTGCTTCGGATGAAGAAATGCTAGCGATTAATCGTGCGCTGGCAATATTTTTAGGGTTTGC
TTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8317.43 Da
• Isoelectric Point: 8.0273

>AT44575 WP_286226489.1 NZ_AP028141:c1293652-1293440 [Polynucleobacter sp. HIN10]
MPVATNLRVQKHRAQLRAAGLRPIQIWVPDTRQSSFAEECRRQSQMLKNDPLESEINEWLDEVRDTKDWK

Nucleotide

• Length: 213 bp

>AT44575 NZ_AP028141:c1293652-1293440 [Polynucleobacter sp. HIN10]
ATGCCAGTTGCCACTAACTTAAGAGTCCAAAAACACCGGGCGCAATTACGTGCGGCAGGGCTTCGTCCTATCCAAATCTG
GGTTCCAGATACACGCCAATCCTCTTTTGCAGAGGAGTGTCGCCGTCAGTCGCAAATGTTAAAAAATGATCCACTCGAAT
CTGAAATCAATGAGTGGCTTGATGAGGTTCGTGATACCAAGGATTGGAAGTGA