Detailed information of TA system TA044561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafN(antitoxin)
• Location: 869420..869985
• Replicon: chromosome
• Accession: NZ_AP028128
• Organism: Vibrio fluvialis strain IDH05335

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QUF32_RS04090
• Protein ID: WP_143683507.1
• Coordinates: 869698..869985 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U3BLE1
• Locus tag: QUF32_RS04085
• Protein ID: WP_000086647.1
• Coordinates: 869420..869701 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF32_RS04065 (KKIDH5335_07480)	866265..866564	+	300	Protein_746	IS5/IS1182 family transposase	-
QUF32_RS04070 (KKIDH5335_07490)	866623..867234	-	612	WP_286236087.1	mobile mystery protein B	-
QUF32_RS04075 (KKIDH5335_07500)	867231..867719	-	489	WP_170905695.1	mobile mystery protein A	-
QUF32_RS04080	868551..869258	-	708	Protein_749	IS110 family transposase	-
QUF32_RS04085 (KKIDH5335_07520)	869420..869701	+	282	WP_000086647.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUF32_RS04090 (KKIDH5335_07530)	869698..869985	+	288	WP_143683507.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF32_RS04095 (KKIDH5335_07540)	870471..870842	+	372	Protein_752	transposase	-
QUF32_RS04100 (KKIDH5335_07550)	870923..871297	+	375	WP_238844891.1	hypothetical protein	-
QUF32_RS04105 (KKIDH5335_07560)	871294..871620	+	327	WP_004393962.1	IS66 family insertion sequence element accessory protein TnpB	-
QUF32_RS04110 (KKIDH5335_07570)	871736..873334	+	1599	WP_286236088.1	IS66 family transposase	-
QUF32_RS04115 (KKIDH5335_07580)	873405..873731	+	327	Protein_756	DDE-type integrase/transposase/recombinase	-
QUF32_RS04120 (KKIDH5335_07590)	873796..874296	+	501	WP_286236089.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	866424..882209	15785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11009.65 Da
• Isoelectric Point: 5.1098

>T44561 WP_143683507.1 NZ_AP028128:869698-869985 [Vibrio fluvialis]
MKVVWSPLALQKLSDAAEFISLDNPSAAEKWVNEVFDKTELLGSMPEMGRFVPEMPHTNYREIIFGHYRIIYSLSHEIRV
LTVRNCRQVLSEDDV

Nucleotide

• Length: 288 bp

>T44561 NZ_AP028128:869698-869985 [Vibrio fluvialis]
ATGAAAGTCGTTTGGTCTCCATTAGCACTTCAGAAGCTAAGCGATGCGGCGGAATTTATTTCTTTGGATAATCCATCTGC
GGCAGAAAAGTGGGTAAACGAAGTTTTTGACAAAACTGAACTACTTGGAAGTATGCCTGAAATGGGGCGCTTTGTTCCTG
AAATGCCCCATACTAACTATCGTGAAATCATTTTTGGTCACTATCGTATTATCTATAGCTTAAGCCATGAGATCCGCGTA
CTAACAGTTCGTAATTGTCGTCAAGTCTTGTCGGAAGATGACGTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10398.91 Da
• Isoelectric Point: 5.8650

>AT44561 WP_000086647.1 NZ_AP028128:869420-869701 [Vibrio fluvialis]
MSRIHFDQDIQPLSEFRAGVASYIKQINETRRPLVITQRGKGVAVVLDVAEYEAMQEKIELLEEMRTAEAQLASGLGVSN
DDARAQVLGRIKK

Nucleotide

• Length: 282 bp

>AT44561 NZ_AP028128:869420-869701 [Vibrio fluvialis]
ATGAGTCGTATTCACTTCGATCAAGATATTCAGCCGTTATCTGAATTTCGCGCAGGTGTAGCTTCTTACATCAAACAAAT
CAATGAGACACGCCGGCCATTGGTCATAACACAACGTGGTAAAGGTGTAGCTGTTGTGTTAGATGTTGCAGAGTATGAAG
CAATGCAAGAGAAAATCGAATTACTAGAAGAAATGCGCACAGCTGAAGCACAATTAGCTTCAGGCCTTGGTGTTTCAAAT
GATGATGCCCGTGCTCAAGTACTGGGCCGCATTAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4P8G0X0