Detailed information of TA system TA044531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1771..2318
• Replicon: plasmid pJNE132847_2
• Accession: NZ_AP028096
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: JE76HU_RS26730
• Protein ID: WP_250110461.1
• Coordinates: 2040..2318 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A8B4NDS9
• Locus tag: JE76HU_RS26725
• Protein ID: WP_033802255.1
• Coordinates: 1771..2040 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS26720 (1)	1..858	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
JE76HU_RS26725 (1771)	1771..2040	+	270	WP_033802255.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
JE76HU_RS26730 (2040)	2040..2318	+	279	WP_250110461.1	type II toxin-antitoxin system toxin YacB	Toxin
JE76HU_RS26735 (2641)	2641..3219	+	579	WP_235802409.1	hypothetical protein	-
JE76HU_RS26740 (3573)	3573..3908	-	336	WP_001340323.1	hypothetical protein	-
JE76HU_RS26745 (4246)	4246..5226	+	981	WP_033807854.1	plasmid segregation protein ParM	-
JE76HU_RS26750 (5229)	5229..5651	+	423	WP_000774832.1	plasmid partitioning/stability family protein	-
JE76HU_RS26755 (5675)	5675..5896	+	222	WP_113263694.1	hypothetical protein	-
JE76HU_RS26760 (5994)	5994..7010	+	1017	WP_023278197.1	IS110-like element ISShdy1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B	-	1..75901	75901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10790.43 Da
• Isoelectric Point: 8.4615

>T44531 WP_250110461.1 NZ_AP028096:2040-2318 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSVSSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T44531 NZ_AP028096:2040-2318 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGAATTGGTTATTCAGTCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10360.73 Da
• Isoelectric Point: 7.2055

>AT44531 WP_033802255.1 NZ_AP028096:1771-2040 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQFERGDVLPHDMVEFSAAAWRD
EMSRKIADK

Nucleotide

• Length: 270 bp

>AT44531 NZ_AP028096:1771-2040 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CTGCAGGACGTCAGCAATTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATTTTCCGCTGCTGCATGGCGGGAT
GAGATGAGCAGGAAGATTGCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4NDS9