Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 101275..101918 Replicon plasmid pJNE132847_1
Accession NZ_AP028095
Organism Escherichia coli strain JNE132847

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag JE76HU_RS26570 Protein ID WP_001044768.1
Coordinates 101275..101691 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag JE76HU_RS26575 Protein ID WP_001261287.1
Coordinates 101688..101918 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE76HU_RS26550 (96496) 96496..98067 + 1572 WP_000381335.1 IS66-like element ISCro1 family transposase -
JE76HU_RS26555 (98098) 98098..99525 + 1428 Protein_101 IS66-like element ISEc8 family transposase -
JE76HU_RS26560 (99705) 99705..100555 - 851 Protein_102 IS3 family transposase -
JE76HU_RS26565 (100760) 100760..101113 + 354 WP_001427337.1 hypothetical protein -
JE76HU_RS26570 (101275) 101275..101691 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
JE76HU_RS26575 (101688) 101688..101918 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JE76HU_RS26580 (102479) 102479..102892 + 414 WP_000465041.1 hypothetical protein -
JE76HU_RS26585 (102894) 102894..103676 + 783 WP_001164190.1 site-specific integrase -
JE76HU_RS26590 (103848) 103848..104201 + 354 WP_000864811.1 colicin M immunity protein -
JE76HU_RS26595 (104251) 104251..104412 - 162 Protein_109 lipid II-degrading bacteriocin -
JE76HU_RS26600 (104442) 104442..104537 - 96 Protein_110 transcriptional regulator -
JE76HU_RS26605 (104604) 104604..106043 - 1440 WP_001213545.1 enterohemolysin T1SS ABC transporter subunit EhxD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / hlyD / hlyB / hlyA / hlyC 1..132775 132775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T44530 WP_001044768.1 NZ_AP028095:c101691-101275 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T44530 NZ_AP028095:c101691-101275 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT44530 WP_001261287.1 NZ_AP028095:c101918-101688 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT44530 NZ_AP028095:c101918-101688 [Escherichia coli]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAATCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References