Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 66623..67158 Replicon plasmid pJNE132847_1
Accession NZ_AP028095
Organism Escherichia coli strain JNE132847

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag JE76HU_RS26425 Protein ID WP_001270417.1
Coordinates 66623..66910 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag JE76HU_RS26430 Protein ID WP_001132895.1
Coordinates 66907..67158 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 61698..61763 + 66 - - -
JE76HU_RS26390 61751..61900 + 150 Protein_68 plasmid maintenance protein Mok -
JE76HU_RS26395 61842..61967 + 126 WP_001372321.1 type I toxin-antitoxin system Hok family toxin -
JE76HU_RS26400 62258..62638 + 381 WP_238890498.1 transposase -
JE76HU_RS26405 62635..62982 + 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
JE76HU_RS26410 63032..64570 + 1539 WP_000998048.1 IS66-like element ISEc8 family transposase -
JE76HU_RS26415 64720..65696 - 977 Protein_73 RepB family plasmid replication initiator protein -
JE76HU_RS26420 66376..66621 - 246 Protein_74 integrase core domain-containing protein -
JE76HU_RS26425 66623..66910 - 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JE76HU_RS26430 66907..67158 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
JE76HU_RS26435 67874..68683 + 810 Protein_77 AAA family ATPase -
JE76HU_RS26440 68749..69917 + 1169 WP_138039593.1 IS3-like element IS911 family transposase -
JE76HU_RS26445 69927..70331 + 405 Protein_79 ParA family protein -
JE76HU_RS26450 70328..71290 + 963 WP_000756328.1 ParB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 54258..74600 20342
- inside Non-Mobilizable plasmid - toxB / hlyD / hlyB / hlyA / hlyC 1..132775 132775


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T44529 WP_001270417.1 NZ_AP028095:c66910-66623 [Escherichia coli]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T44529 NZ_AP028095:c66910-66623 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGTGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT44529 WP_001132895.1 NZ_AP028095:c67158-66907 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT44529 NZ_AP028095:c67158-66907 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TTGATGCCCTAGACGATCAGGAGTTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGATCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References