Detailed information of TA system TA044525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 38314..38815
• Replicon: plasmid pJNE132847_1
• Accession: NZ_AP028095
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: JE76HU_RS26260
• Protein ID: WP_000702245.1
• Coordinates: 38314..38574 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: JE76HU_RS26265
• Protein ID: WP_001132407.1
• Coordinates: 38564..38815 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS26220 (33735)	33735..34163	-	429	WP_286352590.1	hypothetical protein	-
JE76HU_RS26225 (34552)	34552..35739	-	1188	WP_000937595.1	IS91 family transposase	-
JE76HU_RS26230 (35739)	35739..36104	-	366	WP_032345630.1	hypothetical protein	-
JE76HU_RS26235 (36123)	36123..36413	+	291	WP_170995147.1	hypothetical protein	-
JE76HU_RS26240 (36464)	36464..36838	+	375	WP_021500037.1	hypothetical protein	-
JE76HU_RS26245 (37368)	37368..37691	+	324	WP_000427403.1	hypothetical protein	-
JE76HU_RS26250 (37732)	37732..37941	+	210	WP_000866036.1	hypothetical protein	-
JE76HU_RS26255 (38038)	38038..38262	+	225	WP_000814381.1	hypothetical protein	-
JE76HU_RS26260 (38314)	38314..38574	+	261	WP_000702245.1	hypothetical protein	Toxin
JE76HU_RS26265 (38564)	38564..38815	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
JE76HU_RS26270 (39364)	39364..39642	+	279	WP_000545937.1	hypothetical protein	-
JE76HU_RS26275 (40062)	40062..40328	+	267	WP_000681608.1	type II toxin-antitoxin system HicB family antitoxin	-
JE76HU_RS26280 (40435)	40435..40878	-	444	WP_000068545.1	hypothetical protein	-
JE76HU_RS26285 (41175)	41175..41357	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
JE76HU_RS26290 (41382)	41382..41819	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / hlyD / hlyB / hlyA / hlyC	1..132775	132775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T44525 WP_000702245.1 NZ_AP028095:38314-38574 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T44525 NZ_AP028095:38314-38574 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT44525 WP_001132407.1 NZ_AP028095:38564-38815 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT44525 NZ_AP028095:38564-38815 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7