Detailed information of TA system TA044524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1766..2325
• Replicon: plasmid pJNE132847_1
• Accession: NZ_AP028095
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JE76HU_RS26065
• Protein ID: WP_138039488.1
• Coordinates: 2050..2325 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: JE76HU_RS26060
• Protein ID: WP_001178089.1
• Coordinates: 1766..2050 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS26055 (1)	1..858	+	858	WP_032345402.1	incFII family plasmid replication initiator RepA	-
JE76HU_RS26060 (1766)	1766..2050	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
JE76HU_RS26065 (2050)	2050..2325	+	276	WP_138039488.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JE76HU_RS26070 (2480)	2480..3296	+	817	Protein_4	ABC transporter permease	-
JE76HU_RS26075 (3562)	3562..4581	-	1020	WP_000878012.1	IS110 family transposase	-
JE76HU_RS26080 (4729)	4729..5121	+	393	Protein_6	FtsX-like permease family protein	-
JE76HU_RS26085 (5245)	5245..5682	-	438	WP_138039490.1	YadA-like family protein	-
JE76HU_RS26090 (6003)	6003..6416	+	414	WP_138039491.1	hypothetical protein	-
JE76HU_RS26095 (6418)	6418..7197	+	780	WP_138039493.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / hlyD / hlyB / hlyA / hlyC	1..132775	132775
	-	flank	IS/Tn	-	-	3562..4581	1019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10714.35 Da
• Isoelectric Point: 8.4830

>T44524 WP_138039488.1 NZ_AP028095:2050-2325 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASEPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTVY
VLRLWQTRENR

Nucleotide

• Length: 276 bp

>T44524 NZ_AP028095:2050-2325 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTGAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTCACTGGTCAGACTGTCTAT
GTATTGCGCCTGTGGCAAACGCGGGAAAACAGATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT44524 WP_001178089.1 NZ_AP028095:1766-2050 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT44524 NZ_AP028095:1766-2050 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGAGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8