Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4116379..4116965 Replicon chromosome
Accession NZ_AP028094
Organism Escherichia coli strain JNE132847

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag JE76HU_RS20980 Protein ID WP_042014162.1
Coordinates 4116735..4116965 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JE76HU_RS20975 Protein ID WP_000593555.1
Coordinates 4116379..4116738 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE76HU_RS20950 (4112511) 4112511..4113455 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
JE76HU_RS20955 (4113452) 4113452..4114285 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JE76HU_RS20960 (4114285) 4114285..4115049 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JE76HU_RS20965 (4115046) 4115046..4115852 + 807 WP_024215994.1 nickel import ATP-binding protein NikE -
JE76HU_RS20970 (4115858) 4115858..4116259 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JE76HU_RS20975 (4116379) 4116379..4116738 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JE76HU_RS20980 (4116735) 4116735..4116965 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
JE76HU_RS20985 (4117069) 4117069..4118193 - 1125 WP_001314210.1 ABC transporter permease -
JE76HU_RS20990 (4118193) 4118193..4120928 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T44520 WP_042014162.1 NZ_AP028094:c4116965-4116735 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T44520 NZ_AP028094:c4116965-4116735 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44520 WP_000593555.1 NZ_AP028094:c4116738-4116379 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44520 NZ_AP028094:c4116738-4116379 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References