Detailed information of TA system TA044516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2952763..2953388
• Replicon: chromosome
• Accession: NZ_AP028094
• Organism: Escherichia coli strain JNE132847

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JE76HU_RS14975
• Protein ID: WP_000911330.1
• Coordinates: 2952763..2953161 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JE76HU_RS14980
• Protein ID: WP_000450524.1
• Coordinates: 2953161..2953388 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JE76HU_RS14955 (2948641)	2948641..2948841	+	201	WP_000383836.1	YpfN family protein	-
JE76HU_RS14960 (2948951)	2948951..2949649	-	699	WP_000679823.1	esterase	-
JE76HU_RS14965 (2949723)	2949723..2951738	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
JE76HU_RS14970 (2951753)	2951753..2952616	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
JE76HU_RS14975 (2952763)	2952763..2953161	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
JE76HU_RS14980 (2953161)	2953161..2953388	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JE76HU_RS14985 (2953542)	2953542..2954255	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JE76HU_RS14990 (2954468)	2954468..2955502	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
JE76HU_RS14995 (2955519)	2955519..2956397	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JE76HU_RS15000 (2956543)	2956543..2957115	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JE76HU_RS15005 (2957115)	2957115..2957585	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T44516 WP_000911330.1 NZ_AP028094:c2953161-2952763 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T44516 NZ_AP028094:c2953161-2952763 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCAACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT44516 WP_000450524.1 NZ_AP028094:c2953388-2953161 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT44516 NZ_AP028094:c2953388-2953161 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33