Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 490777..491395 Replicon chromosome
Accession NZ_AP028094
Organism Escherichia coli strain JNE132847

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JE76HU_RS02240 Protein ID WP_001291435.1
Coordinates 490777..490995 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JE76HU_RS02245 Protein ID WP_000344800.1
Coordinates 491021..491395 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JE76HU_RS02205 (486067) 486067..486639 + 573 WP_000779826.1 YbaY family lipoprotein -
JE76HU_RS02210 (486670) 486670..486981 - 312 WP_000409911.1 MGMT family protein -
JE76HU_RS02220 (487360) 487360..487713 + 354 WP_000878141.1 DUF1428 family protein -
JE76HU_RS02225 (487755) 487755..489305 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JE76HU_RS02230 (489469) 489469..489939 - 471 WP_000136192.1 YlaC family protein -
JE76HU_RS02235 (490055) 490055..490606 - 552 WP_000102578.1 maltose O-acetyltransferase -
JE76HU_RS02240 (490777) 490777..490995 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JE76HU_RS02245 (491021) 491021..491395 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JE76HU_RS02250 (491941) 491941..495090 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JE76HU_RS02255 (495113) 495113..496306 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44498 WP_001291435.1 NZ_AP028094:c490995-490777 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44498 NZ_AP028094:c490995-490777 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44498 WP_000344800.1 NZ_AP028094:c491395-491021 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44498 NZ_AP028094:c491395-491021 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References