Detailed information of TA system TA044491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 582573..583062
• Replicon: chromosome
• Accession: NZ_AP028087
• Organism: Burkholderia pseudomallei strain KM390

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q63NA2
• Locus tag: QUF27_RS21395
• Protein ID: WP_004547086.1
• Coordinates: 582573..582794 (+)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: QUF27_RS21400
• Protein ID: WP_004525420.1
• Coordinates: 582778..583062 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF27_RS21355	578221..578649	-	429	Protein_473	integrase core domain-containing protein	-
QUF27_RS21360	578848..579381	-	534	Protein_474	SPFH domain-containing protein	-
QUF27_RS21365 (BpKM390_41160)	579814..580404	+	591	WP_009946442.1	hypothetical protein	-
QUF27_RS21370 (BpKM390_41170)	580579..580797	-	219	WP_004549687.1	hypothetical protein	-
QUF27_RS21375 (BpKM390_41180)	580845..581192	-	348	WP_009946438.1	hypothetical protein	-
QUF27_RS21380 (BpKM390_41190)	581221..581397	-	177	WP_004552958.1	hypothetical protein	-
QUF27_RS21385	581536..581667	-	132	WP_015967385.1	hypothetical protein	-
QUF27_RS21390 (BpKM390_41200)	581677..581952	-	276	WP_004537565.1	bacteriophage protein Gp49	-
QUF27_RS21395 (BpKM390_41210)	582573..582794	+	222	WP_004547086.1	BrnT family toxin	Toxin
QUF27_RS21400 (BpKM390_41220)	582778..583062	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
QUF27_RS21405 (BpKM390_41230)	583909..585003	-	1095	WP_009961431.1	IS5 family transposase	-
QUF27_RS21410 (BpKM390_41240)	585102..585449	+	348	Protein_484	integrase core domain-containing protein	-
QUF27_RS21415 (BpKM390_41250)	585412..585840	+	429	Protein_485	IS110 family transposase	-
QUF27_RS21420 (BpKM390_41260)	586067..586183	-	117	Protein_486	IS3 family transposase	-
QUF27_RS21425 (BpKM390_41280)	586449..587909	+	1461	WP_004547073.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	575910..591558	15648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (14-65)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8634.77 Da
• Isoelectric Point: 8.4920

>T44491 WP_004547086.1 NZ_AP028087:582573-582794 [Burkholderia pseudomallei]
MECRWRLQRNSTGQDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 222 bp

>T44491 NZ_AP028087:582573-582794 [Burkholderia pseudomallei]
ATGGAGTGTCGTTGGCGCTTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAG
TGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGC
ACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44491 WP_004525420.1 NZ_AP028087:582778-583062 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44491 NZ_AP028087:582778-583062 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q63NA2

Antitoxin

• AlphaFold DB: A0A0F6L9S8