Detailed information of TA system TA044490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3971800..3972455
• Replicon: chromosome
• Accession: NZ_AP028086
• Organism: Burkholderia pseudomallei strain KM390

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QUF27_RS18275
• Protein ID: WP_024431052.1
• Coordinates: 3971800..3972096 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: QUF27_RS18280
• Protein ID: WP_004202809.1
• Coordinates: 3972099..3972455 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF27_RS18265 (BpKM390_35280)	3967486..3969132	+	1647	WP_197970186.1	AAA family ATPase	-
QUF27_RS18270 (BpKM390_35290)	3969157..3970869	+	1713	WP_229766272.1	anti-phage dCTP deaminase	-
QUF27_RS18275 (BpKM390_35300)	3971800..3972096	+	297	WP_024431052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF27_RS18280 (BpKM390_35310)	3972099..3972455	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
QUF27_RS18285 (BpKM390_35320)	3972499..3973554	-	1056	WP_044362993.1	phage portal protein	-
QUF27_RS18290 (BpKM390_35330)	3973551..3975320	-	1770	WP_038718265.1	terminase ATPase subunit family protein	-
QUF27_RS18295 (BpKM390_35340)	3975464..3976273	+	810	WP_038718264.1	GPO family capsid scaffolding protein	-
QUF27_RS18300 (BpKM390_35350)	3976307..3977317	+	1011	WP_038718263.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3960721..4005807	45086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10916.71 Da
• Isoelectric Point: 9.9369

>T44490 WP_024431052.1 NZ_AP028086:3971800-3972096 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRREKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T44490 NZ_AP028086:3971800-3972096 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCGATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGAAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT44490 WP_004202809.1 NZ_AP028086:3972099-3972455 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT44490 NZ_AP028086:3972099-3972455 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAATACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3