Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 1232340..1234096 Replicon chromosome
Accession NZ_AP028082
Organism Burkholderia pseudomallei strain Tokushima

Toxin (Protein)


Gene name doc Uniprot ID A0A0F6L2M7
Locus tag QUF25_RS23540 Protein ID WP_024429492.1
Coordinates 1232340..1233245 (+) Length 302 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q63LW2
Locus tag QUF25_RS23545 Protein ID WP_004195450.1
Coordinates 1233242..1234096 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF25_RS23510 (TKS_45170) 1227735..1228823 - 1089 WP_004184835.1 porin -
QUF25_RS23515 (TKS_45180) 1229258..1229575 + 318 WP_004184916.1 non-heme iron oxygenase ferredoxin subunit -
QUF25_RS23520 (TKS_45190) 1229577..1229888 + 312 WP_004184841.1 hypothetical protein -
QUF25_RS23525 (TKS_45200) 1229923..1230990 + 1068 WP_004195457.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
QUF25_RS23530 (TKS_45210) 1230987..1231757 + 771 WP_004524000.1 SDR family oxidoreductase -
QUF25_RS23535 (TKS_45220) 1231796..1232323 + 528 WP_004524001.1 cupin domain-containing protein -
QUF25_RS23540 (TKS_45230) 1232340..1233245 + 906 WP_024429492.1 alpha/beta fold hydrolase Toxin
QUF25_RS23545 (TKS_45240) 1233242..1234096 + 855 WP_004195450.1 IclR family transcriptional regulator Antitoxin
QUF25_RS23550 (TKS_45250) 1234093..1234890 + 798 WP_004528161.1 SDR family oxidoreductase -
QUF25_RS23555 (TKS_45260) 1234883..1236556 + 1674 WP_004524004.1 thiamine pyrophosphate-binding protein -
QUF25_RS23560 (TKS_45270) 1236549..1237364 + 816 WP_004528162.1 aspartate dehydrogenase -
QUF25_RS23565 (TKS_45280) 1237390..1238880 + 1491 WP_004524006.1 aldehyde dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(54-284)

Antitoxin

Transcription regulator IclR, C-terminal

(143-265)


Sequences

Toxin        


Download         Length: 302 a.a.        Molecular weight: 30542.66 Da        Isoelectric Point: 8.4944

>T44486 WP_024429492.1 NZ_AP028082:1232340-1233245 [Burkholderia pseudomallei]
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIERCVLVGHSLGAIMAGAFARIAGERLGGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Download         Length: 906 bp

>T44486 NZ_AP028082:1232340-1233245 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCATCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCGAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCGGGCGAGCGGCTGGGGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCTGCAGGT
GATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCCGTCAAT
GCGACGGATCGCGAGGCACACCATGA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31152.56 Da        Isoelectric Point: 6.8932

>AT44486 WP_004195450.1 NZ_AP028082:1233242-1234096 [Burkholderia pseudomallei]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFDVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR

Download         Length: 855 bp

>AT44486 NZ_AP028082:1233242-1234096 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTCTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCGGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCATTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6L2M7

Antitoxin

Source ID Structure
AlphaFold DB A0A3N4FRW8

References