Detailed information of TA system TA044485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 582336..582768
• Replicon: chromosome
• Accession: NZ_AP028082
• Organism: Burkholderia pseudomallei strain Tokushima

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: QUF25_RS20795
• Protein ID: WP_009946429.1
• Coordinates: 582336..582500 (+)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: QUF25_RS20800
• Protein ID: WP_004525420.1
• Coordinates: 582484..582768 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF25_RS20750	577927..578354	-	428	Protein_473	integrase core domain-containing protein	-
QUF25_RS20755	578553..579086	-	534	Protein_474	SPFH domain-containing protein	-
QUF25_RS20760 (TKS_39930)	579122..579271	-	150	WP_004537646.1	bacteriophage protein Gp44	-
QUF25_RS20765 (TKS_39940)	579521..580111	+	591	WP_009946442.1	hypothetical protein	-
QUF25_RS20770 (TKS_39950)	580286..580504	-	219	WP_004549687.1	hypothetical protein	-
QUF25_RS20775 (TKS_39960)	580552..580899	-	348	WP_009946438.1	hypothetical protein	-
QUF25_RS20780 (TKS_39970)	580928..581104	-	177	WP_004552958.1	hypothetical protein	-
QUF25_RS20785	581243..581374	-	132	WP_015967385.1	hypothetical protein	-
QUF25_RS20790 (TKS_39980)	581384..581659	-	276	WP_004537565.1	bacteriophage protein Gp49	-
QUF25_RS20795 (TKS_39990)	582336..582500	+	165	WP_009946429.1	BrnT family toxin	Toxin
QUF25_RS20800 (TKS_40000)	582484..582768	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
QUF25_RS20805 (TKS_40010)	583615..584709	-	1095	WP_009961431.1	IS5 family transposase	-
QUF25_RS20810 (TKS_40020)	584808..585155	+	348	Protein_485	integrase core domain-containing protein	-
QUF25_RS20815 (TKS_40030)	585118..585546	+	429	Protein_486	IS110 family transposase	-
QUF25_RS20820 (TKS_40040)	585773..585889	-	117	Protein_487	IS3 family transposase	-
QUF25_RS20825 (TKS_40060)	586155..587615	+	1461	WP_004547073.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	575616..591264	15648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-46)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6310.15 Da
• Isoelectric Point: 8.0031

>T44485 WP_009946429.1 NZ_AP028082:582336-582500 [Burkholderia pseudomallei]
VDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 165 bp

>T44485 NZ_AP028082:582336-582500 [Burkholderia pseudomallei]
GTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTT
CACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATGTCGAGCAAG
CGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44485 WP_004525420.1 NZ_AP028082:582484-582768 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44485 NZ_AP028082:582484-582768 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8