Detailed information of TA system TA044484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3864574..3865229
• Replicon: chromosome
• Accession: NZ_AP028081
• Organism: Burkholderia pseudomallei strain Tokushima

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QUF25_RS17670
• Protein ID: WP_024431052.1
• Coordinates: 3864574..3864870 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: QUF25_RS17675
• Protein ID: WP_004202809.1
• Coordinates: 3864873..3865229 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF25_RS17660 (TKS_34000)	3860293..3861906	+	1614	WP_200525244.1	AAA family ATPase	-
QUF25_RS17665 (TKS_34010)	3861931..3863643	+	1713	WP_229766272.1	anti-phage dCTP deaminase	-
QUF25_RS17670 (TKS_34020)	3864574..3864870	+	297	WP_024431052.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF25_RS17675 (TKS_34030)	3864873..3865229	+	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
QUF25_RS17680 (TKS_34040)	3865273..3866328	-	1056	WP_044362993.1	phage portal protein	-
QUF25_RS17685 (TKS_34050)	3866325..3868094	-	1770	WP_038718265.1	terminase ATPase subunit family protein	-
QUF25_RS17690 (TKS_34060)	3868238..3869047	+	810	WP_038718264.1	GPO family capsid scaffolding protein	-
QUF25_RS17695 (TKS_34070)	3869081..3870091	+	1011	WP_038718263.1	phage major capsid protein, P2 family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3853495..3902308	48813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10916.71 Da
• Isoelectric Point: 9.9369

>T44484 WP_024431052.1 NZ_AP028081:3864574-3864870 [Burkholderia pseudomallei]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRREKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T44484 NZ_AP028081:3864574-3864870 [Burkholderia pseudomallei]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCGATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGAAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT44484 WP_004202809.1 NZ_AP028081:3864873-3865229 [Burkholderia pseudomallei]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT44484 NZ_AP028081:3864873-3865229 [Burkholderia pseudomallei]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAATACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAACCCGCGGCTGATTCAAGCCGCGCTCGGAAACGTCGCGAAAGCGCGCGGCATGACAGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTATCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3