Detailed information of TA system TA044481

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 523292..523829
• Replicon: chromosome
• Accession: NZ_AP028080
• Organism: Burkholderia pseudomallei strain Kanagawa

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: QUF28_RS20645
• Protein ID: WP_004549731.1
• Coordinates: 523292..523561 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: QUF28_RS20650
• Protein ID: WP_004525420.1
• Coordinates: 523545..523829 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF28_RS20600	519091..519519	-	429	Protein_429	integrase core domain-containing protein	-
QUF28_RS20605	519718..520251	-	534	Protein_430	SPFH domain-containing protein	-
QUF28_RS20610 (KNG_39410)	520287..520436	-	150	WP_004542626.1	bacteriophage protein Gp44	-
QUF28_RS20615 (KNG_39420)	520692..521273	+	582	WP_004542548.1	hypothetical protein	-
QUF28_RS20620 (KNG_39430)	521504..521722	-	219	WP_004552392.1	hypothetical protein	-
QUF28_RS20625 (KNG_39440)	521770..521928	-	159	WP_004549802.1	hypothetical protein	-
QUF28_RS20630 (KNG_39450)	521925..522167	-	243	WP_004545584.1	hypothetical protein	-
QUF28_RS20635	522305..522436	-	132	WP_009923452.1	bacteriophage protein Gp48	-
QUF28_RS20640 (KNG_39460)	522446..522721	-	276	WP_038716556.1	hypothetical protein	-
QUF28_RS20645 (KNG_39470)	523292..523561	+	270	WP_004549731.1	BrnT family toxin	Toxin
QUF28_RS20650 (KNG_39480)	523545..523829	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
QUF28_RS20660 (KNG_39490)	524476..524859	+	384	Protein_440	integrase core domain-containing protein	-
QUF28_RS20665 (KNG_39500)	524822..525250	+	429	Protein_441	IS110 family transposase	-
QUF28_RS20670 (KNG_39510)	525477..525593	-	117	Protein_442	IS3 family transposase	-
QUF28_RS20675 (KNG_39530)	525840..527300	+	1461	WP_004547073.1	metallophosphoesterase	-
QUF28_RS20680 (KNG_39540)	527459..528721	+	1263	WP_004537187.1	cytochrome c peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	516780..530949	14169

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T44481 WP_004549731.1 NZ_AP028080:523292-523561 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T44481 NZ_AP028080:523292-523561 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT44481 WP_004525420.1 NZ_AP028080:523545-523829 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT44481 NZ_AP028080:523545-523829 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8