Detailed information of TA system TA044465

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-ccdA/YoeB-CcdA
• Location: 4227968..4228605
• Replicon: chromosome
• Accession: NZ_AP028062
• Organism: Marinobacter shengliensis strain D49

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: QUE75_RS19320
• Protein ID: WP_286252638.1
• Coordinates: 4227968..4228222 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: QUE75_RS19325
• Protein ID: WP_286252640.1
• Coordinates: 4228360..4228605 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE75_RS19295 (MAALD49_38400)	4223126..4224895	-	1770	WP_286252630.1	DUF4394 domain-containing protein	-
QUE75_RS19300 (MAALD49_38410)	4225083..4225859	-	777	WP_286252632.1	transporter substrate-binding domain-containing protein	-
QUE75_RS19305 (MAALD49_38420)	4225902..4226771	-	870	WP_286252635.1	LysR family transcriptional regulator	-
QUE75_RS19310 (MAALD49_38430)	4226866..4227366	+	501	WP_106693235.1	lactoylglutathione lyase family protein	-
QUE75_RS19315 (MAALD49_38440)	4227720..4227971	+	252	WP_106693233.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
QUE75_RS19320 (MAALD49_38450)	4227968..4228222	+	255	WP_286252638.1	Txe/YoeB family addiction module toxin	Toxin
QUE75_RS19325 (MAALD49_38460)	4228360..4228605	+	246	WP_286252640.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
QUE75_RS19330 (MAALD49_38470)	4228605..4228922	+	318	WP_203301045.1	CcdB family protein	-
QUE75_RS19335 (MAALD49_38480)	4229004..4229291	-	288	WP_286252644.1	helix-turn-helix domain-containing protein	-
QUE75_RS19340 (MAALD49_38490)	4229284..4229595	-	312	WP_286252646.1	hypothetical protein	-
QUE75_RS19345 (MAALD49_38500)	4229725..4229961	-	237	WP_286252648.1	hypothetical protein	-
QUE75_RS19350 (MAALD49_38510)	4230180..4231085	-	906	WP_286252650.1	toll/interleukin-1 receptor domain-containing protein	-
QUE75_RS19355 (MAALD49_38520)	4231307..4232878	-	1572	WP_286250329.1	transposase	-
QUE75_RS19360 (MAALD49_38530)	4233041..4233346	-	306	WP_286252651.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4229004..4245363	16359
	-	inside	Integron	-	-	4227588..4231085	3497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Post-segregation antitoxin CcdA (11-81)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10256.77 Da
• Isoelectric Point: 10.0153

>T44465 WP_286252638.1 NZ_AP028062:4227968-4228222 [Marinobacter shengliensis]
MKFIFSENAREDYLYWQKTDKRILNRINKLIKETKREPFEGVGKPEPLKHSLAGYWSRRTNEEHRIVYKVTDDALLIAQL
RYHY

Nucleotide

• Length: 255 bp

>T44465 NZ_AP028062:4227968-4228222 [Marinobacter shengliensis]
ATGAAATTCATCTTCTCTGAGAACGCCCGGGAAGACTATCTGTACTGGCAGAAAACCGACAAAAGGATTCTTAACCGAAT
CAACAAGCTGATCAAAGAGACCAAGCGAGAACCGTTCGAAGGTGTTGGCAAACCAGAGCCCCTCAAACACAGCCTCGCCG
GGTACTGGTCTCGCCGAACCAACGAAGAACACCGAATAGTTTACAAAGTCACGGATGACGCTTTGCTTATAGCCCAACTC
CGGTATCACTACTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9260.43 Da
• Isoelectric Point: 6.9810

>AT44465 WP_286252640.1 NZ_AP028062:4228360-4228605 [Marinobacter shengliensis]
MAELYNSAAPKKAANLSINSDLLRKARELNINLSATLERALKEELSKRKAAQWVEENRAAIKSYNDFVEQHGCFGDEFRE
F

Nucleotide

• Length: 246 bp

>AT44465 NZ_AP028062:4228360-4228605 [Marinobacter shengliensis]
ATGGCCGAACTCTATAACTCAGCCGCCCCCAAAAAAGCAGCGAACCTCTCAATCAATAGCGATCTTCTGCGTAAAGCCCG
GGAACTGAACATTAACTTGTCTGCCACCCTGGAACGAGCTCTGAAAGAAGAGCTCTCGAAACGCAAAGCCGCTCAATGGG
TTGAAGAGAATCGCGCTGCGATAAAAAGCTACAACGATTTCGTCGAACAACATGGATGCTTCGGCGACGAATTCAGGGAG
TTCTGA