Detailed information of TA system TA044457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2306864..2307307
• Replicon: chromosome
• Accession: NZ_AP028061
• Organism: Geobacter sulfurreducens subsp. ethanolicus strain OSK2A

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: QUE50_RS10735
• Protein ID: WP_286349831.1
• Coordinates: 2306864..2307004 (+)
• Length: 47 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q74CX9
• Locus tag: QUE50_RS10740
• Protein ID: WP_010942189.1
• Coordinates: 2307014..2307307 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE50_RS10710 (GSUET_20820)	2302908..2303327	-	420	WP_286349827.1	hypothetical protein	-
QUE50_RS10715 (GSUET_20830)	2303431..2304777	-	1347	WP_286349661.1	IS5 family transposase	-
QUE50_RS10720 (GSUET_20840)	2304885..2305244	-	360	WP_286349828.1	hypothetical protein	-
QUE50_RS10725 (GSUET_20850)	2305444..2306058	-	615	WP_286349829.1	LysE family translocator	-
QUE50_RS10730 (GSUET_20860)	2306153..2306599	-	447	WP_286349830.1	DUF2845 domain-containing protein	-
QUE50_RS10735 (GSUET_20870)	2306864..2307004	+	141	WP_286349831.1	hypothetical protein	Toxin
QUE50_RS10740 (GSUET_20880)	2307014..2307307	+	294	WP_010942189.1	putative addiction module antidote protein	Antitoxin
QUE50_RS10745 (GSUET_20890)	2307429..2308799	-	1371	WP_119332507.1	kelch repeat-containing protein	-
QUE50_RS10750 (GSUET_20900)	2308840..2309643	-	804	WP_286349832.1	transposase	-
QUE50_RS10755 (GSUET_20910)	2309848..2311410	-	1563	WP_286349833.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2303431..2304777	1346

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-46)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 47 a.a.
• Molecular weight: 5144.93 Da
• Isoelectric Point: 10.1613

>T44457 WP_286349831.1 NZ_AP028061:2306864-2307004 [Geobacter sulfurreducens subsp. ethanolicus]
MRIDYGPGYRVYFFQRGSELIVLLAGGDKGSQSRDIKAAIRLAQNL

Nucleotide

• Length: 141 bp

>T44457 NZ_AP028061:2306864-2307004 [Geobacter sulfurreducens subsp. ethanolicus]
ATGCGTATCGACTACGGTCCTGGTTACCGAGTTTATTTCTTCCAGCGAGGAAGCGAATTGATCGTCCTGCTGGCCGGCGG
CGATAAAGGCAGCCAGTCTCGGGATATCAAGGCGGCAATCCGCCTCGCACAGAACCTATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10451.92 Da
• Isoelectric Point: 6.4708

>AT44457 WP_010942189.1 NZ_AP028061:2307014-2307307 [Geobacter sulfurreducens subsp. ethanolicus]
MTKTATSRYDVAEHLRTPEEMAAYLEASLEEANGDATFIAKALGDIARAKGMSQVARDAGLSRESLYKALSGERVPSFDT
ILKIIAALGLKLHAEAR

Nucleotide

• Length: 294 bp

>AT44457 NZ_AP028061:2307014-2307307 [Geobacter sulfurreducens subsp. ethanolicus]
ATGACCAAAACCGCAACCAGTCGCTATGACGTCGCCGAACATCTCCGTACTCCGGAGGAAATGGCCGCCTACCTTGAAGC
CTCCCTCGAAGAGGCTAATGGCGATGCGACCTTCATCGCCAAGGCACTCGGAGACATAGCCCGCGCCAAGGGGATGTCGC
AGGTTGCACGAGATGCCGGTCTTTCCCGCGAAAGTCTTTATAAGGCACTTTCAGGAGAGCGGGTTCCCAGCTTTGATACC
ATCCTTAAAATCATTGCGGCACTTGGACTTAAACTGCACGCAGAAGCCAGGTAA

Structures

Antitoxin

• AlphaFold DB: Q74CX9