Detailed information of TA system TA044456

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1331238..1331763
• Replicon: chromosome
• Accession: NZ_AP028061
• Organism: Geobacter sulfurreducens subsp. ethanolicus strain OSK2A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: QUE50_RS06320
• Protein ID: WP_286352003.1
• Coordinates: 1331238..1331528 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q74AD8
• Locus tag: QUE50_RS06325
• Protein ID: WP_010943075.1
• Coordinates: 1331518..1331763 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE50_RS06295 (GSUET_12200)	1327167..1328144	+	978	WP_286351999.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
QUE50_RS06300 (GSUET_12210)	1328278..1328850	+	573	WP_286352000.1	hypothetical protein	-
QUE50_RS06305	1328989..1329459	+	471	WP_286352001.1	AMIN domain-containing protein	-
QUE50_RS06310 (GSUET_12220)	1329899..1330645	+	747	WP_286352002.1	class I SAM-dependent methyltransferase	-
QUE50_RS06315 (GSUET_12230)	1330773..1331189	+	417	WP_010943077.1	hypothetical protein	-
QUE50_RS06320 (GSUET_12240)	1331238..1331528	-	291	WP_286352003.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE50_RS06325 (GSUET_12250)	1331518..1331763	-	246	WP_010943075.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
QUE50_RS06330 (GSUET_12260)	1332141..1332626	+	486	WP_014551816.1	VOC family protein	-
QUE50_RS06335 (GSUET_12270)	1332667..1333653	+	987	WP_286352004.1	alpha-ketoacid dehydrogenase subunit beta	-
QUE50_RS06340 (GSUET_12280)	1333669..1334925	+	1257	WP_286352005.1	dihydrolipoamide acetyltransferase family protein	-
QUE50_RS06345 (GSUET_12290)	1334954..1335661	+	708	WP_286352006.1	lipoyl(octanoyl) transferase LipB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-67)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11321.27 Da
• Isoelectric Point: 10.4995

>T44456 WP_286352003.1 NZ_AP028061:c1331528-1331238 [Geobacter sulfurreducens subsp. ethanolicus]
MSYSLEFKESALKEWKKLDGRIREQFKKKLTERLELPRVESARLSGMPDCYKIKLKNAGYRLVYQVDDNRIIVTVVAVGK
RENLTVYRAAEKRVKQ

Nucleotide

• Length: 291 bp

>T44456 NZ_AP028061:c1331528-1331238 [Geobacter sulfurreducens subsp. ethanolicus]
ATGAGCTATAGCCTCGAATTCAAGGAATCCGCCCTGAAGGAGTGGAAAAAACTCGATGGCAGAATCCGTGAACAGTTTAA
GAAGAAGCTTACCGAACGGCTTGAGCTTCCTCGCGTCGAGTCGGCCCGACTCTCCGGCATGCCCGATTGCTACAAAATCA
AGCTGAAGAACGCCGGCTACCGGCTGGTTTACCAGGTTGACGACAACCGCATCATCGTTACTGTCGTGGCTGTCGGAAAA
CGCGAAAACCTTACCGTTTATCGAGCGGCAGAGAAAAGGGTGAAACAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9193.46 Da
• Isoelectric Point: 4.6631

>AT44456 WP_010943075.1 NZ_AP028061:c1331763-1331518 [Geobacter sulfurreducens subsp. ethanolicus]
MERLFARASVSISDLKKNPTRIINQSEGEPVAILNHNKPSAYLIPADAFEALMEKLEDYELSQIVKEREHEPSVKVSLDE
L

Nucleotide

• Length: 246 bp

>AT44456 NZ_AP028061:c1331763-1331518 [Geobacter sulfurreducens subsp. ethanolicus]
ATGGAACGTCTTTTTGCCCGTGCCTCGGTCAGCATAAGTGACCTTAAAAAAAACCCCACCCGAATCATTAATCAGTCCGA
AGGTGAACCTGTGGCAATCCTCAACCACAACAAGCCAAGTGCATACTTAATCCCCGCCGATGCCTTCGAGGCGCTCATGG
AGAAACTGGAGGATTATGAGCTTTCCCAAATTGTGAAGGAACGGGAACACGAGCCGAGTGTCAAGGTCTCCCTCGATGAG
CTATAG

Structures

Antitoxin

• AlphaFold DB: Q74AD8