Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 519972..520608 | Replicon | chromosome |
| Accession | NZ_AP028059 | ||
| Organism | Bacillus subtilis subsp. natto strain NARUSE | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | QUE82_RS02655 | Protein ID | WP_003156187.1 |
| Coordinates | 520258..520608 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | QUE82_RS02650 | Protein ID | WP_003225183.1 |
| Coordinates | 519972..520253 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QUE82_RS02630 (BSNN_04870) | 516331..516930 | - | 600 | WP_014478896.1 | rhomboid family intramembrane serine protease | - |
| QUE82_RS02635 (BSNN_04880) | 517025..517390 | + | 366 | WP_014478897.1 | holo-ACP synthase | - |
| QUE82_RS02640 (BSNN_04890) | 517556..518572 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| QUE82_RS02645 (BSNN_04900) | 518687..519856 | + | 1170 | WP_014478898.1 | alanine racemase | - |
| QUE82_RS02650 (BSNN_04910) | 519972..520253 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| QUE82_RS02655 (BSNN_04920) | 520258..520608 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| QUE82_RS02660 (BSNN_04930) | 520724..521548 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| QUE82_RS02665 (BSNN_04940) | 521553..521918 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| QUE82_RS02670 (BSNN_04950) | 521922..522323 | + | 402 | WP_014478899.1 | serine/threonine-protein kinase RsbT | - |
| QUE82_RS02675 (BSNN_04960) | 522335..523342 | + | 1008 | WP_014478900.1 | phosphoserine phosphatase RsbU | - |
| QUE82_RS02680 (BSNN_04970) | 523411..523740 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| QUE82_RS02685 (BSNN_04980) | 523737..524219 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| QUE82_RS02690 (BSNN_04990) | 524185..524973 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| QUE82_RS02695 (BSNN_05000) | 524973..525572 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T44453 WP_003156187.1 NZ_AP028059:520258-520608 [Bacillus subtilis subsp. natto]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T44453 NZ_AP028059:520258-520608 [Bacillus subtilis subsp. natto]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT44453 WP_003225183.1 NZ_AP028059:519972-520253 [Bacillus subtilis subsp. natto]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT44453 NZ_AP028059:519972-520253 [Bacillus subtilis subsp. natto]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|