Detailed information of TA system TA044448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5277500..5278163
• Replicon: chromosome
• Accession: NZ_AP028050
• Organism: Phytobacter diazotrophicus strain TA9832

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A348DL83
• Locus tag: QUE83_RS25360
• Protein ID: WP_039077908.1
• Coordinates: 5277500..5277844 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A348DL84
• Locus tag: QUE83_RS25365
• Protein ID: WP_071194355.1
• Coordinates: 5277846..5278163 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE83_RS25340 (5273177)	5273177..5274583	-	1407	WP_039077905.1	replicative DNA helicase	-
QUE83_RS25345 (5274642)	5274642..5275625	+	984	WP_039077906.1	quinone oxidoreductase	-
QUE83_RS25350 (5275914)	5275914..5276156	-	243	WP_039077907.1	envelope stress response protein PspG	-
QUE83_RS25355 (5276309)	5276309..5277307	-	999	WP_039077935.1	tRNA dihydrouridine(20/20a) synthase DusA	-
QUE83_RS25360 (5277500)	5277500..5277844	+	345	WP_039077908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE83_RS25365 (5277846)	5277846..5278163	+	318	WP_071194355.1	helix-turn-helix transcriptional regulator	Antitoxin
QUE83_RS25370 (5278205)	5278205..5279521	-	1317	WP_039077910.1	conjugal transfer protein TraF	-
QUE83_RS25375 (5279794)	5279794..5280309	+	516	WP_039077912.1	zinc uptake transcriptional repressor Zur	-
QUE83_RS25380 (5280417)	5280417..5280626	-	210	WP_039077936.1	CsbD family protein	-
QUE83_RS25385 (5280724)	5280724..5280912	+	189	WP_071194358.1	hypothetical protein	-
QUE83_RS25390 (5280887)	5280887..5281501	-	615	WP_039077914.1	transcriptional repressor LexA	-
QUE83_RS25395 (5281610)	5281610..5281978	-	369	WP_079497650.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12655.46 Da
• Isoelectric Point: 9.5820

>T44448 WP_039077908.1 NZ_AP028050:5277500-5277844 [Phytobacter diazotrophicus]
MKPLYWVGSSKKDLQSLPEDVQDVFGYALHLAQDGGKHPQAKPLKGFGGAGVLEVVEDYCTDTYRAVYTLKFRQAVYVLH
AFQKKSSSGMQTPKSDIEKIRERLKAAEAHARGA

Nucleotide

• Length: 345 bp

>T44448 NZ_AP028050:5277500-5277844 [Phytobacter diazotrophicus]
ATGAAGCCACTGTATTGGGTGGGGAGCAGCAAAAAAGATCTTCAGTCTCTGCCGGAGGATGTTCAGGATGTTTTTGGTTA
TGCGTTGCATCTCGCACAAGACGGAGGTAAGCATCCCCAGGCAAAACCGTTAAAAGGATTTGGGGGCGCAGGTGTGCTGG
AGGTGGTCGAAGACTATTGCACCGATACTTATCGCGCGGTTTATACCCTGAAATTCAGACAGGCGGTCTATGTGCTACAT
GCATTTCAGAAGAAGTCGTCATCCGGTATGCAAACCCCCAAATCTGATATAGAAAAAATTCGAGAACGTCTTAAGGCAGC
AGAAGCCCATGCCAGAGGAGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11644.64 Da
• Isoelectric Point: 11.1929

>AT44448 WP_071194355.1 NZ_AP028050:5277846-5278163 [Phytobacter diazotrophicus]
MSQNIDISSGNVYADIGLDNADEMQVKAQLATTIGNIIKSRRLTQVEAAKLLGMTQPRLSNMLRGQFRGISEVKMLECLT
RLGRDVQIVVGRPRRTPGRMKVVFA

Nucleotide

• Length: 318 bp

>AT44448 NZ_AP028050:5277846-5278163 [Phytobacter diazotrophicus]
ATGAGCCAGAATATCGATATCAGCAGCGGCAATGTTTATGCCGACATTGGACTGGACAATGCGGATGAAATGCAGGTTAA
AGCGCAGCTTGCTACCACCATTGGCAATATCATTAAAAGTCGTCGTCTTACTCAGGTGGAGGCGGCAAAGTTATTGGGGA
TGACACAGCCCAGGCTATCAAATATGCTGCGCGGCCAATTTCGCGGCATAAGTGAAGTAAAAATGCTTGAATGTCTGACA
CGCTTAGGGCGCGATGTTCAAATTGTGGTTGGGCGCCCGCGCCGTACCCCGGGACGTATGAAGGTGGTTTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A348DL83

Antitoxin

• AlphaFold DB: A0A348DL84