Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1952204..1952794 Replicon chromosome
Accession NZ_AP028050
Organism Phytobacter diazotrophicus strain TA9832

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag QUE83_RS09125 Protein ID WP_125124239.1
Coordinates 1952462..1952794 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag QUE83_RS09120 Protein ID WP_125124238.1
Coordinates 1952204..1952461 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE83_RS09095 (1947811) 1947811..1948146 - 336 WP_041851877.1 gamma-glutamylcyclotransferase family protein -
QUE83_RS09100 (1948273) 1948273..1948563 - 291 WP_079496374.1 DUF3601 domain-containing protein -
QUE83_RS09105 (1948821) 1948821..1949360 - 540 WP_079496546.1 ester cyclase -
QUE83_RS09110 (1949659) 1949659..1950768 - 1110 WP_125124236.1 alkene reductase -
QUE83_RS09115 (1950869) 1950869..1951780 + 912 WP_125124237.1 LysR family transcriptional regulator -
QUE83_RS09120 (1952204) 1952204..1952461 + 258 WP_125124238.1 antitoxin Antitoxin
QUE83_RS09125 (1952462) 1952462..1952794 + 333 WP_125124239.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QUE83_RS09135 (1953195) 1953195..1954628 + 1434 WP_103245488.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
QUE83_RS09145 (1954913) 1954913..1955140 - 228 WP_125124240.1 hypothetical protein -
QUE83_RS09150 (1955357) 1955357..1956817 + 1461 WP_125124241.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1934201..2033606 99405


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(5-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11781.49 Da        Isoelectric Point: 6.9812

>T44435 WP_125124239.1 NZ_AP028050:1952462-1952794 [Phytobacter diazotrophicus]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSPASFNALTRLPVVVPVTSGGNFARAAGFAVSLEGAGTKTTGVIRCDQPRTI
DMDARNDKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T44435 NZ_AP028050:1952462-1952794 [Phytobacter diazotrophicus]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCGACCACCGGGCATGAACAAAGCGGAAAACGTCCAGTGCTTAT
CGTTTCTCCCGCGTCATTTAATGCACTCACCCGTTTGCCCGTTGTTGTTCCGGTCACCAGTGGCGGAAACTTTGCCCGAG
CGGCGGGTTTTGCCGTCTCACTGGAAGGTGCGGGAACGAAAACCACGGGTGTGATTCGCTGCGATCAGCCCAGAACCATT
GATATGGACGCCCGAAACGACAAGCGTCTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGTTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9272.74 Da        Isoelectric Point: 4.5799

>AT44435 WP_125124238.1 NZ_AP028050:1952204-1952461 [Phytobacter diazotrophicus]
MYIARLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIENGCLIIEPQKRPHYSLEELLAQCDPHAEISEEDRQWIDGPAA
GKELL

Download         Length: 258 bp

>AT44435 NZ_AP028050:1952204-1952461 [Phytobacter diazotrophicus]
ATGTATATCGCTCGCCTTAAAAAGGTTGGAGGATCCGTCATGCTGGCGGTTCCTCCTGCCGTATTGAAAACCCTGGGGCT
GTCGACGGATAGCGAAGTGGGTATGACCATTGAGAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCACATTACTCGC
TTGAGGAATTGCTGGCGCAGTGTGATCCGCACGCCGAAATCAGCGAAGAGGATCGGCAATGGATTGATGGGCCTGCGGCG
GGCAAGGAGCTTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References